Kaposi's Sarcoma Cells: Long-Term Culture with Growth Factor from Retrovirus-Infected CD4 + T Cells
- 21 October 1988
- journal article
- Published by American Association for the Advancement of Science (AAAS) in Science
- Vol. 242 (4877), 426-430
- https://doi.org/10.1126/science.3262925
Abstract
Studies of the biology and pathogenesis of Kaposi's sarcoma (KS) have been hampered by the inability to maintain long-term cultures of KS cells in vitro. In this study AIDS-KS-derived cells with characteristic spindle-like morphology were cultured with a growth factor (or factors) released by CD4+ T lymphocytes infected with human T-lymphotropic virus type I or II (HTLV-I or HTLV-II) or with human immunodeficiency virus type 1 or 2 (HIV-1 or HIV-2). Medium conditioned by HTLV-II-infected, transformed lines of T cells (HTLV-II CM) contained large amounts of this growth activity and also supported the temporary growth of normal vascular endothelial cells, but not fibroblasts. Interleukin-1 and tumor necrosis factor-alpha stimulated the growth of the KS-derived cells, but the growth was only transient and these could be distinguished from that in HTLV-II CM. Other known endothelial cell growth promoting factors, such as acidic and basic fibroblast growth factors and epidermal growth factor, did not support the long-term growth of the AIDS-KS cells. The factor released by CD4+ T cells infected with human retroviruses should prove useful in studies of the pathogenesis of KS.Keywords
This publication has 57 references indexed in Scilit:
- New human and simian HIV-related retroviruses possess functional transactivator (tat) geneNature, 1987
- Synapses that Compute MotionScientific American, 1987
- Genetic Analysis of a New Subgroup of Human and Simian T-Lymphotropic Retroviruses: HTLV-IV, LAV-2, SBL-6669, and STLV-IIIAGMAIDS Research and Human Retroviruses, 1987
- A Second Isolate of HTLV-II Associated with Atypical Hairy-Cell LeukemiaNew England Journal of Medicine, 1986
- Human tumour necrosis factor: precursor structure, expression and homology to lymphotoxinNature, 1984
- Cloning and expression of cDNA for human lymphotoxin, a lymphokine with tumour necrosis activityNature, 1984
- Isolation of a T-Lymphotropic Retrovirus from a Patient at Risk for Acquired Immune Deficiency Syndrome (AIDS)Science, 1983
- Synthesis of alpha and gamma interferons by a human cutaneous lymphoma with helper T-cell phenotypeCellular Immunology, 1982
- Kaposi's Sarcoma: A Review and Recent DevelopmentsCA: A Cancer Journal for Clinicians, 1981
- KAPOSIʼS SARCOMA AFTER KIDNEY TRANSPLANTATION REPORT OF COMPLETE REMISSION OF CUTANEOUS AND VISCERAL INVOLVEMENTTransplantation, 1980