Paraoxonase and Superoxide Dismutase Gene Polymorphisms and Noise-Induced Hearing Loss
- 1 November 2004
- journal article
- Published by Oxford University Press (OUP) in Clinical Chemistry
- Vol. 50 (11), 2012-2018
- https://doi.org/10.1373/clinchem.2004.037788
Abstract
Background: Noise-induced cochlear epithelium damage can cause hearing loss in industrial workers. In experimental systems, noise induces the release of free radicals and may damage the cochlear sensorial epithelium. Therefore, genes involved in regulating the reactive oxygen species manganese-superoxide dismutase (SOD2) and the antioxidant paraoxonase (PON) could influence cochlea vulnerability to noise. We evaluated whether susceptibility to noise-induced hearing loss (NIHL) is associated with SOD2, PON1, and PON2 polymorphisms in workers exposed to prolonged loud noise. Methods: We enrolled 94 male workers from an aircraft factory in the study. The SOD2 gene was screened by denaturing reversed-phase HPLC, and the PON1 (Q192R and M55L) and PON2 (S311C) polymorphisms were analyzed by PCR amplification followed by digestion with restriction endonucleases. Results: Three known (A16V, IVS3-23T/G, and IVS3-60T/G) and two new SOD2 polymorphisms (IVS1+ 8A/G and IVS3+107T/A) were identified. Regression analysis showed that PON2 (SC+CC) [odds ratio (OR) = 5.01; 95% confidence interval (CI), 1.11–22.54], SOD2 IVS3-23T/G and IVS3-60T/G (OR = 5.09; 95% CI, 1.27–20.47), age (OR = 1.22; 95% CI, 1.09–1.36), and smoking (OR = 49.49; 95% CI, 5.09–480.66) were associated with NIHL. No association was detected for PON1 (QQ+RR) and PON1 (LL) genotypes. Conclusions: Our data suggest that SOD2 and PON2 polymorphisms, by exerting variable local tissue antioxidant roles, could predispose to NIHL. However, caution should be exercised in interpreting these data given the small sample size and the difficulty in matching cases to controls regarding the overwhelming risk factor, i.e., smoking at least 10 cigarettes/day.Keywords
This publication has 30 references indexed in Scilit:
- Cigarette smoking, occupational exposure to noise, and self reported hearing difficultiesOccupational and Environmental Medicine, 2004
- Identification of paraoxonase 3 gene (PON3) missense mutations in a population of southern ItalyMutation Research - Reviews in Mutation Research, 2004
- Combined effect of smoking and occupational exposure to noise on hearing loss in steel factory workersOccupational and Environmental Medicine, 2003
- Further evidence for an association of the Paraoxonase 1 (PON1) Met‐54 allele with Parkinson's diseaseMovement Disorders, 2002
- Genetic polymorphisms of superoxide dismutase in Parkinson's diseaseMovement Disorders, 2001
- Nomenclature for the description of human sequence variationsHuman Genetics, 2001
- Early Elevation of Cochlear Reactive Oxygen Species following Noise ExposureAudiology and Neurotology, 1999
- Targeted Deletion of the Cytosolic Cu/Zn-Superoxide Dismutase Gene (Sod1) Increases Susceptibility to Noise-Induced Hearing LossAudiology and Neurotology, 1999
- Analysis of Mitochondrial Targeting Sequence and Coding Region Polymorphisms of the Manganese Superoxide Dismutase Gene in German Parkinson Disease PatientsBiochemical and Biophysical Research Communications, 1999
- Molecular Structure and Organization of the Human Manganese Superoxide Dismutase GeneDNA and Cell Biology, 1994