The Spt-Ada-Gcn5 Acetyltransferase (SAGA) Complex in Aspergillus nidulans
Open Access
- 7 June 2013
- journal article
- research article
- Published by Public Library of Science (PLoS) in PLOS ONE
- Vol. 8 (6), e65221
- https://doi.org/10.1371/journal.pone.0065221
Abstract
A mutation screen in Aspergillus nidulans uncovered mutations in the acdX gene that led to altered repression by acetate, but not by glucose. AcdX of A. nidulans is highly conserved with Spt8p of Saccharomyces cerevisiae, and since Spt8p is a component of the Spt-Ada-Gcn5 Acetyltransferase (SAGA) complex, the SAGA complex may have a role in acetate repression in A. nidulans. We used a bioinformatic approach to identify genes encoding most members of the SAGA complex in A. nidulans, and a proteomic analysis to confirm that most protein components identified indeed exist as a complex in A. nidulans. No apparent compositional differences were detected in mycelia cultured in acetate compared to glucose medium. The methods used revealed apparent differences between Yeast and A. nidulans in the deubiquitination (DUB) module of the complex, which in S. cerevisiae consists of Sgf11p, Sus1p, and Ubp8p. Although a convincing homologue of S. cerevisiae Ubp8p was identified in the A. nidulans genome, there were no apparent homologues for Sus1p and Sgf11p. In addition, when the SAGA complex was purified from A. nidulans, members of the DUB module were not co-purified with the complex, indicating that functional homologues of Sus1p and Sgf11p were not part of the complex. Thus, deubiquitination of H2B-Ub in stress conditions is likely to be regulated differently in A. nidulans compared to S. cerevisiae.Keywords
This publication has 37 references indexed in Scilit:
- Bacteria-induced natural product formation in the fungus Aspergillus nidulans requires Saga/Ada-mediated histone acetylationProceedings of the National Academy of Sciences of the United States of America, 2011
- Sgf29 binds histone H3K4me2/3 and is required for SAGA complex recruitment and histone H3 acetylationThe EMBO Journal, 2011
- Nucleosome Positioning and Histone H3 Acetylation Are Independent Processes in the Aspergillus nidulans prnD-prnB Bidirectional PromoterEukaryotic Cell, 2008
- The SAGA continues: expanding the cellular role of a transcriptional co-activator complexOncogene, 2007
- An eight-subunit COP9 signalosome with an intact JAMM motif is required for fungal fruit body formationProceedings of the National Academy of Sciences of the United States of America, 2007
- Transcriptome Analysis of Aspergillus nidulans Exposed to Camptothecin-Induced DNA DamageEukaryotic Cell, 2006
- The mRNA Export Factor Sus1 Is Involved in Spt/Ada/Gcn5 Acetyltransferase-mediated H2B Deubiquitinylation through Its Interaction with Ubp8 and Sgf11Molecular Biology of the Cell, 2006
- SAGA binds TBP via its Spt8 subunit in competition with DNA: implications for TBP recruitmentThe EMBO Journal, 2006
- Ubp8p, a Histone Deubiquitinase Whose Association with SAGA Is Mediated by Sgf11p, Differentially Regulates Lysine 4 Methylation of Histone H3 In VivoMolecular and Cellular Biology, 2006
- The language of covalent histone modificationsNature, 2000