Simultaneous Determination of Pyridoxine Hydrochloride and Doxylamine Succinate from Tablets by Ion Pair Reversed-Phase High-Performance Liquid Chromatography (RP-HPLC)
- 1 January 1999
- journal article
- Published by Taylor & Francis Ltd in Drug Development and Industrial Pharmacy
- Vol. 25 (8), 945-950
- https://doi.org/10.1081/ddc-100102255
Abstract
A new, simple, precise, and rapid ion pair reversed-phase high-performance liquid chromatography (RP-HPLC) method has been developed for the simultaneous determination of pyridoxine hydrochloride (PYR) and doxylamine succinate (DOX) in tablets. The stationary phase was a Microbondapak C18 column (10 μ, 300 mm × 3.9 mm i.d.). The mobile phase was water:methanol (60:40) containing 10 mM heptanesulfonic acid and 0.25% triethylamine and adjusted to pH 2.2 with orthophosphoric acid. Detection was carried out at 263 nm using an ultraviolet (UV) detector. The flow rate was 1.0 ml/min, and retention times were 3.65 min and 7.32 min for PYR and DOX, respectively. The linearity was obtained in the concentration range 0.5–500 μg/ml for PYR and DOX. Mean percentage recoveries were 100.20% and 101.20% for PYR and DOX, respectively.Keywords
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