The zinc finger of Eco1 enhances its acetyltransferase activity during sister chromatid cohesion
Open Access
- 19 August 2009
- journal article
- research article
- Published by Oxford University Press (OUP) in Nucleic Acids Research
- Vol. 37 (18), 6126-6134
- https://doi.org/10.1093/nar/gkp656
Abstract
Eco1p/Ctf7p is an essential acetyltransferase required for the establishment of sister chromatid cohesion. Eco1p acetylates Smc3p and Mcd1p (Scc1p or Rad21p) to establish cohesion during S phase and in response to DNA damage, respectively. In addition to its acetyltransferase domain, Eco1p harbors a conserved zinc finger domain. The zinc finger has been implicated in the establishment of sister chromatid cohesion in S phase, yet its function on the molecular level and its contribution to damage-induced cohesion are unknown. Here, we show that the zinc finger is essential for the establishment of cohesion in both S phase and in response to DNA damage. Our results suggest that the zinc finger augments the acetylation of Eco1p itself, Smc3p and likely Mcd1p. We propose that the zinc finger is a general enhancer of substrate recognition, thereby enhances the ability of Eco1p to acetylate its substrates above a threshold needed to generate cohesion during DNA replication and repair. Finally our studies of the zinc finger led to the discovery that Eco1 is a multimer, a property that could be exploited to coordinate acetylation of substrates either spatially or temporally for establishment of sister chromatid cohesion.This publication has 45 references indexed in Scilit:
- Building Sister Chromatid Cohesion: Smc3 Acetylation Counteracts an Antiestablishment ActivityMolecular Cell, 2009
- A handcuff model for the cohesin complexThe Journal of cell biology, 2008
- Sister Chromatid Cohesion: A Simple Concept with a Complex RealityAnnual Review of Cell and Developmental Biology, 2008
- The Human Monocytic Leukemia Zinc Finger Histone Acetyltransferase Domain Contains DNA-binding Activity Implicated in Chromatin TargetingJournal of Biological Chemistry, 2007
- PCNA Controls Establishment of Sister Chromatid Cohesion during S PhaseMolecular Cell, 2006
- Regulation of the Drosophila transcription factor, Cubitus interruptus, by two conserved domainsDevelopmental Biology, 2006
- Eco1 Is a Novel Acetyltransferase that Can Acetylate Proteins Involved in CohesionCurrent Biology, 2002
- Solution structure of the TAZ2 (CH3) domain of the transcriptional adaptor protein CBPJournal of Molecular Biology, 2000
- Crystal structure of the histone acetyltransferase Hpa2: a tetrameric member of the Gcn5-related N-acetyltransferase superfamilyJournal of Molecular Biology, 1999
- Comparative Protein Modelling by Satisfaction of Spatial RestraintsJournal of Molecular Biology, 1993