Formation of droplet networks that function in aqueous environments
- 6 November 2011
- journal article
- research article
- Published by Springer Science and Business Media LLC in Nature Nanotechnology
- Vol. 6 (12), 803-808
- https://doi.org/10.1038/nnano.2011.183
Abstract
Aqueous droplets in oil that are coated with lipid monolayers and joined through interface bilayers1,2 are useful for biophysical measurements on membrane proteins2,3,4,5. Functional networks of droplets that can act as light sensors, batteries and electrical components can also be made by incorporating pumps, channels and pores into the bilayers2,6. These networks of droplets mimic simple tissues7, but so far have not been used in physiological environments because they have been constrained to a bulk oil phase. Here, we form structures called multisomes in which networks of aqueous droplets with defined compositions are encapsulated within small drops of oil in water. The encapsulated droplets adhere to one another and to the surface of the oil drop to form interface bilayers that allow them to communicate with each other and with the surrounding aqueous environment through membrane pores. The contents in the droplets can be released by changing the pH or temperature of the surrounding solution. The multicompartment framework of multisomes mimics a tissue7,8,9 and has potential applications in synthetic biology and medicine.Keywords
This publication has 35 references indexed in Scilit:
- Structural and functional studies of gap junction channelsCurrent Opinion in Structural Biology, 2010
- Analysis of Single Nucleic Acid Molecules with Protein NanoporesMethods in Enzymology, 2010
- Convection-enhanced delivery for the treatment of brain tumorsExpert Review of Neurotherapeutics, 2009
- DNA Strands from Denatured Duplexes are Translocated through Engineered Protein Nanopores at Alkaline pHNano Letters, 2009
- Droplet networks with incorporated protein diodes show collective propertiesNature Nanotechnology, 2009
- Single-nucleotide discrimination in immobilized DNA oligonucleotides with a biological nanoporeProceedings of the National Academy of Sciences of the United States of America, 2009
- Screening Blockers Against a Potassium Channel with a Droplet Interface Bilayer ArrayJournal of the American Chemical Society, 2008
- Synthetic biology through biomolecular design and engineeringCurrent Opinion in Structural Biology, 2008
- Synthetic protocell biology: from reproduction to computationPhilosophical Transactions Of The Royal Society B-Biological Sciences, 2007
- Lipid Bilayer Formation by Contacting Monolayers in a Microfluidic Device for Membrane Protein AnalysisAnalytical Chemistry, 2006