INFLUENCE OF GLUCAGON, AN INDUCER OF CELLULAR AUTOPHAGY, ON SOME PHYSICAL PROPERTIES OF RAT LIVER LYSOSOMES
Open Access
- 1 May 1967
- journal article
- research article
- Published by Rockefeller University Press in The Journal of cell biology
- Vol. 33 (2), 437-449
- https://doi.org/10.1083/jcb.33.2.437
Abstract
The response of rat liver lysosomes to an intraperitoneal injection of glucagon has been evaluated from studies on the mechanical fragility, osmotic sensitivity, and sedimentation properties of these subcellular particles. It has been found that about ½ hr after the injection of glucagon the hepatic lysosomes exhibit a fairly sudden increase in their sensitivity to mechanical stresses and to exposure to a decreased osmotic pressure. At the same time, their sedimentation properties undergo complex changes characterized mainly by a significant increase in the sedimentation coefficient of a considerable proportion of the total particles. In addition, glucagon causes an increase in the proportion of slowly sedimenting particles, with the result that the distribution of sedimentation coefficients within the total population tends to become bimodal. The latter change is more pronounced for acid phosphatase, less so for cathepsin D, and barely detectable for acid deoxyribonuclease. All these modifications are maximal between 45 and 90 min after injection and regress to normal within approximately 4 hr. With the exception of the increase in the slow component, for which no explanation can be advanced at the present time, they are consistent with the hypothesis that glucagon causes an increase in lysosomal size, and may be related to the autophagic-vacuole formation known to occur after glucagon administration.Keywords
This publication has 12 references indexed in Scilit:
- LYSOSOMES IN LYMPHOID TISSUEThe Journal of cell biology, 1967
- Functions of LysosomesAnnual Review of Physiology, 1966
- Tissue fractionation studies. 18. Resolution of mitochondrial fractions from rat liver into three distinct populations of cytoplasmic particles by means of density equilibration in various gradientsBiochemical Journal, 1964
- CYTOPLASMIC COMPONENTS IN HEPATIC CELL LYSOSOMESThe Journal of cell biology, 1962
- Tissue fractionation studies. 12. Intracellular distribution of some dehydrogenases, alkaline deoxyribonuclease and iron in rat-liver tissueBiochemical Journal, 1959
- CELLULAR DIFFERENTIATION IN THE KIDNEYS OF NEWBORN MICE STUDIED WITH THE ELECTRON MICROSCOPEThe Journal of cell biology, 1957
- Tissue fractionation studies. 6. Intracellular distribution patterns of enzymes in rat-liver tissueBiochemical Journal, 1955
- A METHOD FOR ISOLATING INTACT MITOCHONDRIA AND NUCLEI FROM THE SAME HOMOGENATE, AND THE INFLUENCE OF MITOCHONDRIAL DESTRUCTION ON THE PROPERTIES OF CELL NUCLEIThe Journal of cell biology, 1955
- Tissue fractionation studies. 3. Further observations on the binding of acid phosphatase by rat-liver particlesBiochemical Journal, 1955
- Tissue fractionation studies. 4. Comparative study of the binding of acid phosphatase, β-glucuronidase and cathepsin by rat-liver particlesBiochemical Journal, 1955