Hydrolysis of Nonstarch Carbohydrates of Wheat‐Starch Effluent for Ethanol Production
- 1 January 2004
- journal article
- research article
- Published by Wiley in Biotechnology Progress
- Vol. 20 (2), 474-479
- https://doi.org/10.1021/bp034243h
Abstract
A (polysaccharide-rich) waste stream derived from a combined starch and ethanol factory was investigated regarding hydrolysis of the nonstarch carbohydrates for ethanol production. The material was characterized and processed to yield the maximum amount of sugars. The starch fraction was hydrolyzed with amylolytic enzymes, and the resulting fibrous material was separated by filtration. This material, denoted starch-free fibers (SFF), was subjected to heat treatment followed by enzymatic hydrolysis to recover the other major carbohydrate components, namely, cellulose and hemicellulose, in monomeric form. Heat treatment in a microwave oven efficiently solubilized a fraction of these polysaccharides and made the material more accessible to the cellulolytic and hemicellulolytic enzymes used in the subsequent enzymatic hydrolysis. The maximum sugar yield after enzymatic hydrolysis, achieved with pretreatment at 170 degrees C for 40 min, was 34.1 g per 100 g SFF, comprising 12.8 g glucose, 13.9 g xylose and 7.4 g arabinose, corresponding to 66%, 71% and 51% of the theoretical, respectively.Keywords
This publication has 15 references indexed in Scilit:
- Enzymatic hydrolysis of water‐soluble wheat arabinoxylan. 1. Synergy between α‐L‐arabinofuranosidases, endo‐1,4‐β‐xylanases, and β‐xylosidase activitiesBiotechnology & Bioengineering, 2003
- Dilute-Sulfuric Acid Pretreatment of Corn Stover in Pilot-Scale Reactor: Investigation of Yields, Kinetics, and Enzymatic Digestibilities of SolidsApplied Biochemistry and Biotechnology, 2003
- Hydrothermal Pretreatment Conditions to Enhance Ethanol Production from Poplar BiomassApplied Biochemistry and Biotechnology, 2003
- Ethanol Production at Flask and Pilot Scale from Concentrated Slurries of Steam-Exploded AspenIndustrial & Engineering Chemistry Research, 2002
- Two-Step Steam Pretreatment of Softwood with SO2 Impregnation for Ethanol ProductionApplied Biochemistry and Biotechnology, 2002
- Hydrolysis of lignocellulosic materials for ethanol production: a reviewBioresource Technology, 2001
- Production of ethanol from wet oxidised wheat straw by Thermoanaerobacter mathraniiBioresource Technology, 1999
- The generation of fermentation inhibitors during dilute acid hydrolysis of softwoodEnzyme and Microbial Technology, 1999
- Ethanol from lignocellulosics: A review of the economyBioresource Technology, 1996
- Inhibition of Trichoderma reesei cellulase by sugars and solventsBiotechnology & Bioengineering, 1990