Identification of proteins differentially expressed during chondrogenesis of mesenchymal cells
- 10 March 2004
- journal article
- research article
- Published by Wiley in FEBS Letters
- Vol. 563 (1-3), 35-40
- https://doi.org/10.1016/s0014-5793(04)00243-1
Abstract
We performed comparative proteome analysis of mesenchymal cells and chondrocytes to identify proteins differentially expressed during chondrogenesis. Nine such proteins were identified. Type II collagen, matrilin‐1, carbonic anhydrase‐II (CA‐II), 3′‐phosphoadenosine 5′‐phosphosulfate (PAPS) synthetase‐2, and aldo‐keto reductase were increased during chondrogenesis, whereas cellular retinoic acid binding protein‐I (CRABP‐I), CRABP‐II, cytoplasmic type 5 actin, and fatty acid binding protein were decreased or almost disappeared. Expression of type II collagen, matrilin‐1, PAPS synthetase‐2, and CA‐II was regulated by extracellular signal‐regulated protein kinase, protein kinase C, and p38 kinase, signaling molecules known to regulate chondrogenesis.Keywords
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