Time profile of calcium accumulation in hippocampus, striatum and frontoparietal cortex after transient forebrain ischemia in the gerbil
- 1 September 1992
- journal article
- Published by Springer Science and Business Media LLC in Acta Neuropathologica
- Vol. 84 (4), 400-406
- https://doi.org/10.1007/bf00227667
Abstract
The topical and temporal relationship between neuronal injury and calcium loading was investigated in gerbils following bilateral carotid artery occlusion for 5 or 10 min and recirculation times from 15 min to 7 days. The association of histochemically visible calcium deposits with neuronal death was assessed by combining two calcium stains, alizarin red and arsenazo III, with conventional histological techniques. Neuronal calcium accumulation was evaluated morphometrically in the striatum, the frontoparietal cortex and the CA1 and CA4 sectors of the hippocampus. After 5-min ischemia and 1–2 days of recirculation numerous calcium-containing neurons appeared in the CA4 sector but only a few were present in the CA1 sector. After 4 days of recirculation calcium accumulation was visible in the whole CA1 sector and the dorso-lateral part of striate nucleus. After 10-min ischemia calcium accumulation started in these regions, as well as in the cortex, already after 1 day. In the CA1 sector calcium accumulation followed a typical time course: on day 2 only the lateral parts were affected, while on day 4 the whole CA1 neuronal band was calcium positive. The regional distribution of histological lesions matched that of calcium loading and, furthermore, the lesions appeared after a corresponding delay in the respective regions. Morphometric evaluations of calcium staining and histological lesions in the CA1 sector revealed a high correlation, indicating that calcium accumulation and neuronal death are closely associated both topically and temporally. This suggests that disturbances of calcium homeostasis such as those measured by this histochemical technique are the consequence of and not the reason for ischemic cell death.Keywords
This publication has 27 references indexed in Scilit:
- Phenytoin affects metabolism of free fatty acids and nucleotides in rat cerebral ischemia.Stroke, 1990
- Protection by NMDA Antagonists against Selective Cell Loss following Transient IschaemiaJournal of Cerebral Blood Flow & Metabolism, 1990
- Immunohistochemical Determination of Calcium—Calmodulin Binding Predicts Neuronal Damage after Global IschemiaJournal of Cerebral Blood Flow & Metabolism, 1989
- Calcium accumulation by glutamate receptor activation is involved in hippocampal cell damage after ischemiaActa Neurologica Scandinavica, 1988
- Efficacy and mechanism of action of a calcium channel blocker after global cerebral ischemia in rats.Stroke, 1988
- The N-methyl-d-aspartate antagonists CGS 19755 and CPP reduce ischemic brain damage in gerbilsBrain Research, 1988
- Calcium in the mitochondria following brief ischemia of gerbil brainNeuroscience Letters, 1987
- The effect of the calcium antagonist nimodipine on the gerbil model of experimental cerebral ischemia.Stroke, 1986
- Uptake of radiolabeled ions in normal and ischemia‐damaged brainAnnals of Neurology, 1986
- Calcium Dependence of Toxic Cell Death: A Final Common PathwayScience, 1979