Placental, Renal, and Ileal Sulfate Transporter Gene Expression in Mouse Gestation1
- 1 August 2012
- journal article
- Published by Oxford University Press (OUP) in Biology of Reproduction
- Vol. 87 (2), 43
- https://doi.org/10.1095/biolreprod.111.098749
Abstract
Sulfate is important for mammalian growth and development. During pregnancy, maternal circulating sulfate levels increase by 2-fold, enhancing sulfate availability to the fetus. We used quantitative real-time PCR to determine sulfate transporter mRNA levels during mouse gestation in three tissues: kidney and ileum, to identify transporters involved in sulfate absorption and maintaining high maternal circulating sulfate level; and placenta, to build a model of directional sulfate transport from mother to fetus. In the kidney, Slc13a1 and Slc26a1 were the most abundant sulfate transporter mRNAs, which increased by ≈2-fold at E4.5 or E6.5, whereas lower levels of Slc26a2, Slc26a6, and Slc26a7 mRNA increased by ≈3- to 6-fold from E4.5. Ileal sulfate transporter mRNA levels were not increased in gestation, but slight decreases (by ≈30-40%) were found for Slc26a3 and Slc26a6. In placentae, Slc13a4 and Slc26a2 mRNAs were most abundant, with levels increasing from E10.5 and peaking (≈8-fold) from E14.5 to E18.5, whereas Slc26a1 increased by ≈3-fold at E18.5. The spatial expression of placental mRNAs was determined by in situ hybridization showing Slc13a4 and Slc26a6 in yolk sac, Slc26a1 in spongiotrophoblasts, and Slc13a4, Slc26a2, Slc26a3, and Slc26a7 in the labyrinthine layer. Within the labyrinth, cell-specific staining revealed Slc13a4 expression in syncytiotrophoblast-II (SynT-II) and Slc26a2 in SynT-I. Together, these data show kidney Slc13a1 and Slc26a1 and placental Slc13a4 and Slc26a2 to be the most abundant sulfate transporter mRNAs in mouse gestation, which likely play important physiological roles in maintaining high maternal serum sulfate levels during pregnancy and mediating sulfate supply to the fetus.Keywords
This publication has 40 references indexed in Scilit:
- Sulfate in fetal developmentSeminars in Cell & Developmental Biology, 2011
- Steroid metabolome in fetal and maternal body fluids in human late pregnancyThe Journal of Steroid Biochemistry and Molecular Biology, 2010
- The Roles of Chondroitin-4-Sulfotransferase-1 in Development and DiseasePublished by Elsevier BV ,2010
- InactivatingPAPSS2Mutations in a Patient with Premature PubarcheThe New England Journal of Medicine, 2009
- Interindividual variability in acetaminophen sulfation by human fetal liver: Implications for pharmacogenetic investigations of drug‐induced birth defectsBirth Defects Research Part A: Clinical and Molecular Teratology, 2008
- Loss of chondroitin 6-O-sulfotransferase-1 function results in severe human chondrodysplasia with progressive spinal involvementProceedings of the National Academy of Sciences of the United States of America, 2004
- Sulfation pattern in glycosaminoglycan: Does it have a code?Glycoconjugate Journal, 2004
- Regulation of thyroid hormone metabolism during fetal developmentMolecular and Cellular Endocrinology, 1999
- Mutations in orthologous genes in human spondyloepimetaphyseal dysplasia and the brachymorphic mouseNature Genetics, 1998
- Sulfation of endogenous compounds and xenobiotics — interactions and function in health and diseaseChemico-Biological Interactions, 1994