Abstract
A biohydrogen production system coupling the polysaccharide such as sucrose and maltose degradation with invertase and glucose dehydrognase (GDH) and hydrogen production with colloidal platinum as hydrogen‐evolved catalyst using the visible light‐induced photosensitization of water‐soluble zinc porphyrin, zinc tetraphenylporphyrin tetrasulfonate (ZnTPPS) has been investigated. Continuous hydrogen gas production was observed when the sample solution containing polysaccharide, invertase, GDH, nicotinamide adenine dinucreotide (NAD+), ZnTPPS, methylviologen (an electron relay reagent), and colloidal platinum was irradiated by visible light. After 240‐min irradiation, the amount of hydrogen production in the system using sucrose and maltose was estimated to be 3.1 and 0.35 μmol, respectively. © 2003 Wiley Periodicals, Inc. Biotechnol Bioeng 82: 710–714, 2003.