Cross‐blocking studies with monoclonal antibodies against I‐A molecules of haplotypes b, d and k

Abstract

For assessment of the distribution of allodeterminants on I‐A molecules, binding inhibition studies were performed with monoclonal anti‐I‐A antibodies (mAb) in which pairs of labeled and unlabeled mAb were tested for cross‐blocking on spleen cells of H‐2 haplotypes b, d and k. The data suggest that allodeterminants are randomly distributed on the surface of the I‐A molecule. Comparison of mAb with cross‐reactivity for b, d or k haplotypes indicates that the allelic forms of determinants are located in analogous positions on different I‐A antigens.