Modulation of CRISPR locus transcription by the repeat-binding protein Cbp1 in Sulfolobus
Open Access
- 1 December 2011
- journal article
- research article
- Published by Oxford University Press (OUP) in Nucleic Acids Research
- Vol. 40 (6), 2470-2480
- https://doi.org/10.1093/nar/gkr1111
Abstract
CRISPR loci are essential components of the adaptive immune system of archaea and bacteria. They consist of long arrays of repeats separated by DNA spacers encoding guide RNAs (crRNA), which target foreign genetic elements. Cbp1 (CRISPR DNA repeat binding protein) binds specifically to the multiple direct repeats of CRISPR loci of members of the acidothermophilic, crenarchaeal order Sulfolobales. cbp1 gene deletion from Sulfolobus islandicus REY15A produced a strong reduction in pre-crRNA yields from CRISPR loci but did not inhibit the foreign DNA targeting capacity of the CRISPR/Cas system. Conversely, overexpression of Cbp1 in S. islandicus generated an increase in pre-crRNA yields while the level of reverse strand transcripts from CRISPR loci remained unchanged. It is proposed that Cbp1 modulates production of longer pre-crRNA transcripts from CRISPR loci. A possible mechanism is that it minimizes interference from potential transcriptional signals carried on spacers deriving from A-T-rich genetic elements and, occasionally, on DNA repeats. Supporting evidence is provided by microarray and northern blotting analyses, and publicly available whole-transcriptome data for S. solfataricus P2.Keywords
This publication has 43 references indexed in Scilit:
- Macromolecular Fingerprinting of Sulfolobus Species in Biofilm: A Transcriptomic and Proteomic Approach Combined with Spectroscopic AnalysisJournal of Proteome Research, 2011
- CRISPR-based adaptive immune systemsCurrent Opinion in Microbiology, 2011
- Structural and Functional Characterization of an Archaeal Clustered Regularly Interspaced Short Palindromic Repeat (CRISPR)-associated Complex for Antiviral Defense (CASCADE)Online Journal of Public Health Informatics, 2011
- Evolution and classification of the CRISPR–Cas systemsNature Reviews Microbiology, 2011
- CRISPR RNA maturation by trans-encoded small RNA and host factor RNase IIINature, 2011
- Interaction of the Cas6 Riboendonuclease with CRISPR RNAs: Recognition and CleavageStructure, 2011
- Structural and Functional Studies of Archaeal VirusesOnline Journal of Public Health Informatics, 2009
- Cas6 is an endoribonuclease that generates guide RNAs for invader defense in prokaryotesGenes & Development, 2008
- CRISPRcompar: a website to compare clustered regularly interspaced short palindromic repeatsNucleic Acids Research, 2008
- Genes with internal repeats require the THO complex for transcriptionProceedings of the National Academy of Sciences of the United States of America, 2006