Extending Energy-Filtered Transmission Electron Microscopy (EFTEM) into Three Dimensions Using Electron Tomography
- 1 August 2003
- journal article
- microscopy techniques
- Published by Oxford University Press (OUP) in Microscopy and Microanalysis
- Vol. 9 (6), 542-555
- https://doi.org/10.1017/s1431927603030162
Abstract
The length scales on which materials microstructures are being formed, grown, and even designed are becoming increasingly small and increasingly three-dimensional. For such complex structures two-dimensional transmission electron microscopy (TEM) analysis is often inadequate and occasionally misleading. One approach to this problem is the modification of electron tomography techniques, developed for structural biology, for use in materials science. Energy-Filtered (EF) TEM elemental distribution images approximate to true projections of structure, and, as such, can be used to reconstruct the three-dimensional distribution of chemical species. A sample holder has been modified to allow the high tilt (±60°) required for tomography and a semiautomatic acquisition script designed to manage energy-loss acquisition. Tilt series data sets have been acquired from two widely different experimental systems, Cr carbides in 316 stainless steel and magnetite nanocrystals in magnetotactic bacteria, demonstrating single- and multiple-element tomography. It is shown that both elemental maps and jump-ratio images are suitable for reconstruction, despite the effects of diffraction contrast in the former and thickness changes in the latter. It is concluded that the image contrast, signal, and signal-to-noise ratio (SNR) are key to the achievable reconstruction quality and, as such, the technique may be of limited value for high energy loss/small inelastic cross section edges.Keywords
This publication has 25 references indexed in Scilit:
- Z-Contrast tomography: a technique in three-dimensional nanostructural analysis based on Rutherford scatteringChemical Communications, 2001
- Three-Dimensional Transmission Electron Microscopy: A Novel Imaging and Characterization Technique with Nanometer Scale Resolution for Materials ScienceThe Journal of Physical Chemistry B, 2000
- Improving image quality by zero‐loss energy filtering: quantitative assessment by means of image cross‐correlationJournal of Microscopy, 1998
- Perspectives of Molecular and Cellular Electron TomographyJournal of Structural Biology, 1997
- Spatial resolution in EFTEM elemental mapsJournal of Microscopy, 1995
- Three‐Dimensional reconstruction of single particles from random and nonrandom tilt seriesJournal of Electron Microscopy Technique, 1988
- Iterative methods for the three-dimensional reconstruction of an object from projectionsJournal of Theoretical Biology, 1972
- ART and science or conditions for three-dimensional reconstruction from electron microscope imagesJournal of Theoretical Biology, 1971
- Algebraic Reconstruction Techniques (ART) for three-dimensional electron microscopy and X-ray photographyJournal of Theoretical Biology, 1970
- The reconstruction of a three-dimensional structure from projections and its application to electron microscopyProceedings of the Royal Society of London. Series A. Mathematical and Physical Sciences, 1970