A mechanism for ramified rolling circle amplification
Open Access
- 7 December 2010
- journal article
- Published by Springer Science and Business Media LLC in BMC Molecular Biology
- Vol. 11 (1), 94
- https://doi.org/10.1186/1471-2199-11-94
Abstract
Amplification of single-stranded DNA circles has wide utility for a variety of applications. The two-primer ramified rolling circle amplification (RAM) reaction provides exponential DNA amplification under isothermal conditions, creating a regular laddered series of double-stranded DNA products. However, the molecular mechanism of the RAM reaction remains unexplained.Keywords
This publication has 19 references indexed in Scilit:
- Quantitative analysis of zeptomole microRNAs based on isothermal ramification amplificationRNA, 2009
- qpcR: an R package for sigmoidal model selection in quantitative real-time polymerase chain reaction analysisBioinformatics, 2008
- Highly accurate sigmoidal fitting of real-time PCR data by introducing a parameter for asymmetryBMC Bioinformatics, 2008
- Nucleic Acid Isothermal Amplification Technologies—A ReviewNucleosides, Nucleotides and Nucleic Acids, 2008
- Use of Ramification Amplification Assay for Detection of Escherichia coli O157:H7 and Other E. coli Shiga Toxin-Producing StrainsJournal of Clinical Microbiology, 2005
- Comprehensive Algorithm for Quantitative Real-Time Polymerase Chain ReactionJournal of Computational Biology, 2005
- Multiplexed genotyping with sequence-tagged molecular inversion probesNature Biotechnology, 2003
- Detection of Chlamydia trachomatis by Isothermal Ramification Amplification Method: a Feasibility StudyJournal of Clinical Microbiology, 2002
- A new mathematical model for relative quantification in real-time RT-PCRNucleic Acids Research, 2001
- Padlock Probes: Circularizing Oligonucleotides for Localized DNA DetectionScience, 1994