Molecular pathways of early CD105-positive erythroid cells as compared with CD34-positive common precursor cells by flow cytometric cell-sorting and gene expression profiling
Open Access
- 11 January 2013
- journal article
- research article
- Published by Springer Science and Business Media LLC in Blood Cancer Journal
- Vol. 3 (1), e100
- https://doi.org/10.1038/bcj.2012.45
Abstract
Special attention has recently been drawn to the molecular network of different genes that are responsible for the development of erythroid cells. The aim of the present study was to establish in detail the immunophenotype of early erythroid cells and to compare the gene expression profile of freshly isolated early erythroid precursors with that of the CD34-positive (CD34+) compartment. Multiparameter flow cytometric analyses of human bone marrow mononuclear cell fractions (n=20) defined three distinct early erythroid stages. The gene expression profile of sorted early erythroid cells was analyzed by Affymetrix array technology. For 4524 genes, a differential regulation was found in CD105-positive erythroid cells as compared with the CD34+ progenitor compartment (2362 upregulated genes). A highly significant difference was observed in the expression level of genes involved in transcription, heme synthesis, iron and mitochondrial metabolism and transforming growth factor-β signaling. A comparison with recently published data showed over 1000 genes that as yet have not been reported to be upregulated in the early erythroid lineage. The gene expression level within distinct pathways could be illustrated directly by applying the Ingenuity software program. The results of gene expression analyses can be seen at the Gene Expression Omnibus repository.Keywords
This publication has 54 references indexed in Scilit:
- EuroFlow antibody panels for standardized n-dimensional flow cytometric immunophenotyping of normal, reactive and malignant leukocytesLeukemia, 2012
- FOG-1 and GATA-1 act sequentially to specify definitive megakaryocytic and erythroid progenitorsThe EMBO Journal, 2011
- Genome-wide Analysis of Simultaneous GATA1/2, RUNX1, FLI1, and SCL Binding in Megakaryocytes Identifies Hematopoietic RegulatorsDevelopmental Cell, 2011
- Tumor-Suppressive Functions of Leucine Zipper Transcription Factor–Like 1Cancer Research, 2010
- Resolving the distinct stages in erythroid differentiation based on dynamic changes in membrane protein expression during erythropoiesisProceedings of the National Academy of Sciences of the United States of America, 2009
- E2A proteins maintain the hematopoietic stem cell pool and promote the maturation of myelolymphoid and myeloerythroid progenitorsProceedings of the National Academy of Sciences of the United States of America, 2009
- Endoglin expression in blood and endothelium is differentially regulated by modular assembly of the Ets/Gata hemangioblast codeBlood, 2008
- Hepcidin regulation: ironing out the detailsJCI Insight, 2007
- Modulation of bone morphogenetic protein signaling in vivo regulates systemic iron balanceJCI Insight, 2007
- BMP4, SCF, and hypoxia cooperatively regulate the expansion of murine stress erythroid progenitorsBlood, 2007