Is IP-10 a Better Biomarker for Active and Latent Tuberculosis in Children than IFNγ?
Open Access
- 9 December 2008
- journal article
- research article
- Published by Public Library of Science (PLoS) in PLOS ONE
- Vol. 3 (12), e3901
- https://doi.org/10.1371/journal.pone.0003901
Abstract
The blood based interferon-gamma release assays (IGRA) for the diagnosis of tuberculosis do not discriminate between active TB disease and latent TB infection (LTBI). The search for distinguishing biomarkers therefore continues, as the accurate diagnosis of tuberculosis is particularly challenging in children. IFN-γ-inducible protein 10 (IP-10/CXCL10) has recently been evaluated as a marker for active TB in adults with promising results. To investigate this new biomarker for active TB and LTBI in paediatrics. We measured IP-10 levels using ELISA in supernatants of whole blood samples stimulated with TB-specific-antigens and negative control antigen. IP-10 is produced in high levels following mycobacterial antigen stimulation in active TB (n = 17) and LTBI (n = 16) compared to controls (n = 16) and to IFN-γ. The baseline levels of IP-10 are increased in active TB and in LTBI, but there is no significant difference of stimulated levels of IP-10 between active TB and LTBI. IP-10 is a biomarker for tuberculosis in children. However like IFNγ, IP-10 also does not distinguish between active TB and LTBI.Keywords
This publication has 24 references indexed in Scilit:
- Improving T-Cell Assays for the Diagnosis of Latent TB Infection: Potential of a Diagnostic Test Based on IP-10PLOS ONE, 2008
- Comparison of two interferon gamma release assays in the diagnosis of Mycobacterium tuberculosis infection and disease in The GambiaBMC Infectious Diseases, 2007
- Interferon- Release Assays Improve the Diagnosis of Tuberculosis and Nontuberculous Mycobacterial Disease in Children in a Country with a Low Incidence of TuberculosisClinical Infectious Diseases, 2007
- Diagnostic and Management Challenges for Childhood Tuberculosis in the Era of HIVThe Journal of Infectious Diseases, 2007
- T cell-based diagnosis of childhood tuberculosis infectionCurrent Opinion in Infectious Diseases, 2007
- IL-23 and IL-17 in the establishment of protective pulmonary CD4+ T cell responses after vaccination and during Mycobacterium tuberculosis challengeNature Immunology, 2007
- Performance of a whole blood interferon gamma assay for detecting latent infection with Mycobacterium tuberculosis in childrenThorax, 2006
- IFN-γ-inducible protein 10 and pentraxin 3 plasma levels are tools for monitoring inflammation and disease activity in Mycobacterium tuberculosis infectionMicrobes and Infection, 2005
- Interferon-γ assays in the immunodiagnosis of tuberculosis: a systematic reviewThe Lancet Infectious Diseases, 2004
- Lymphocyte-specific chemokine receptor CXCR3: regulation, chemokine binding and gene localizationEuropean Journal of Immunology, 1998