Screening for differentially expressed genes between left- and right-sided colon carcinoma by microarray analysis
Open Access
- 18 June 2013
- journal article
- Published by Spandidos Publications in Oncology Letters
- Vol. 6 (2), 353-358
- https://doi.org/10.3892/ol.2013.1414
Abstract
Left-sided colon carcinoma (LSCC) and right‑sided colon carcinoma (RSCC) differ in their genetic susceptibilities to neoplastic transformation. The present study identified 11 genes that were differentially expressed in LSCC and RSCC by expression profiling with microarray analysis. Compared with RSCC, the human genes for L‑lactate dehydrogenase B chain (LDHB), cyclin‑dependent kinase 4 inhibitor D (CDKN2D), phosphatidylinositol‑4‑phosphate‑3‑kinase C2 domain‑containing subunit α (PI3KC2α), protocadherin fat 1 (FAT; a human protein that closely resembles the Drosophila tumor suppressor, fat) and dual specificity protein phosphatase 2 (DUSP2) were upregulated in LSCC. By contrast, genes for ubiquitin D (UBD), casein kinase‑1 binding protein (CK1BP), synaptotagmin‑13 (SYT1), zinc finger protein 560 (ZNF560), pleckstrin homology domain-containing family B member 2 (PLEKHB2) and IgGFc‑binding protein (FCGBP) were downregulated in LSCC compared with RSCC. A quantitative polymerase chain reaction (qPCR) analysis revealed that the mRNA levels of UBD and CK1BP in LSCC were significantly lower compared with those in RSCC (P=0.033 and P=0.005, respectively), whereas the mRNA levels of LDHB and CDKN2D in LSCC were significantly higher compared with those in RSCC (P=0.008 and P=0.017, respectively). Western blot and immunohistochemical analyses demonstrated that the expression of CDKN2D in LSCC was significantly higher compared with that in RSCC, while the expression of UBD in LSCC was significantly lower compared with that in RSCC. The present study provides important insights into the understanding of the molecular genetic basis for the different biological behaviors observed between LSCC and RSCC. These insights may therefore serve as a basis for the identification of novel colon cancer markers and therapeutic targets.Keywords
This publication has 20 references indexed in Scilit:
- Ubiquitin D is correlated with colon cancer progression and predicts recurrence for stage II-III disease after curative surgeryBritish Journal of Cancer, 2010
- Clinicopathological differences between right- and left-sided colonic tumors and impact upon survivalTechniques in Coloproctology, 2010
- Comparison of 17,641 Patients With Right- and Left-Sided Colon Cancer: Differences in Epidemiology, Perioperative Course, Histology, and SurvivalDiseases of the Colon & Rectum, 2010
- Tumor Subsite Location Within the Colon Is Prognostic for Survival After Colon Cancer DiagnosisDiseases of the Colon & Rectum, 2009
- Scoring mechanisms of p16INK4a immunohistochemistry based on either independent nucleic stain or mixed cytoplasmic with nucleic expression can significantly signal to distinguish between endocervical and endometrial adenocarcinomas in a tissue microarray studyJournal of Translational Medicine, 2009
- Altering trend of clinical characteristics of colorectal cancer: a report of 3,607 cases.2009
- The impact of tumor location on the histopathologic expression of colorectal cancer.2007
- Differential expression of IgG Fc binding protein (FcgammaBP) in human normal thyroid tissue, thyroid adenomas and thyroid carcinomasJournal of Endocrinology, 2002
- C-KI-RAS activation and the biological behaviour of proximal and distal colonic adenocarcinomasEuropean Journal Of Cancer, 1996
- Colorectal Cancer: Evidence for Distinct Genetic Categories Based on Proximal or Distal Tumor LocationAnnals of Internal Medicine, 1990