Movement of Retinal Along the Visual Transduction Path
- 23 June 2000
- journal article
- other
- Published by American Association for the Advancement of Science (AAAS) in Science
- Vol. 288 (5474), 2209-2212
- https://doi.org/10.1126/science.288.5474.2209
Abstract
Movement of the ligand/receptor complex in rhodopsin (Rh) has been traced. Bleaching of diazoketo rhodopsin (DK-Rh) containing 11- cis -3-diazo-4-oxo-retinal yields batho-, lumi-, meta-I-, and meta-II-Rh intermediates corresponding to those of native Rh but at lower temperatures. Photoaffinity labeling of DK-Rh and these bleaching intermediates shows that the ionone ring cross-links to tryptophan-265 on helix F in DK-Rh and batho-Rh, and to alanine-169 on helix D in lumi-, meta-I-, and meta-II-Rh intermediates. It is likely that these movements involving a flip-over of the chromophoric ring trigger changes in cytoplasmic membrane loops resulting in heterotrimeric guanine nucleotide–binding protein (G protein) activation.Keywords
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