PROTECTIVE EFFECT OF PRESERVATION OF CANINE PANCREAS BY THE TWO-LAYER (UNIVERSITY OF WISCONSIN SOLUTION/PERFLUOROCHEMICAL) METHOD AGAINST REWARMING ISCHEMIC INJURY DURING IMPLANTATION
Rewarming ischemia during implantation severely compromises posttransplant pancreas graft survival because the graft has already been subjected to warm and cold ischemia before implantation. The purpose of this study was to examine whether preservation of the pancreas graft by the two-layer method ameliorates rewarming ischemic injury of the graft during implantation using a canine model. After flushing with cold University of Wisconsin solution (UW), the pancreas grafts were preserved by the two-layer (UW/ perfluorochemical [PFC]) method (group 1) or simple cold storage in UW (group 2) for 24 hr and then auto-transplanted. In control, the pancreas grafts were flushed out with cold UW and immediately autotrans-planted without preservation (group 3). After completion of vascular anastomosis, vascular clamp was not released until 90, 120, or 150 min of rewarming ischemia, including anastomosis time, had elasped. After 90 min of rewarming ischemia, graft survival rates were 5/5, 100%, 5/5, 100%, and 5/5, 100%, in groups 1, 2, and 3, respectively. After 120 min, all the grafts in groups 2 and 3 failed (0/5, 0%, and 0/5, 0%, respectively); however, all the grafts in group 1 survived (5/5, 100%). Even after 150 min, 1 of 3 grafts in group 1 survived (1/3, 33%). After 24 hr preservation, tissue ATP levels of the grafts in group 1 were about 2-fold the reference values before harvesting (8.23±0.72 vs. 4.44±0.49 μmol/g dry weight, P<0.05) and significantly higher compared with group 2 (8.23±0.72 vs. 1.76±0.52 μmol/g dry weight, P<0.01). After 120 min of rewarming ischemia, tissue ATP levels in group 1 were 84% of the reference values and significantly higher compared with group 2 (3.75±0.25 vs. 1.57±0.48 μmol/g dry weight, P<0.05). Two hours after reperfusion, ATP levels in group 1 were 42% of reference values but significantly higher compared with group 2 (1.86±0.36 vs. 1.03±0.18 μmol/g dry weight, P<0.05). We conclude that the two-layer (UW/PFC) method ameliorates rewarming ischemic injury of the pancreas graft during implantation by increasing tissue ATP contents during preservation and consequently maintaining tissue ATP levels during implantation.