Development and application of a direct radioimmunoassay for aldosterone in saliva
- 30 November 1985
- journal article
- Published by Elsevier BV in Steroids
- Vol. 46 (4-5), 845-855
- https://doi.org/10.1016/0039-128x(85)90033-9
Abstract
A previously described direct radioimmunoassay for plasma aldosterone has been modified to enable direct measurement of the steroid in saliva. The specificity of the method has been demonstrated by assay after high pressure liquid chromatographic purification of saliva extracts. Assay of matched plasma and saliva samples taken from normal subjects during unrestricted and controlled sodium intakes, either under basal conditions or while undergoing ACTH stimulation or dexamethasone suppression, confirms that salivary aldosterone values provide a good reflection of levels in plasma. Mean salivary aldosterone values are approximately one-third of those in plasma. Sampling immediately upon waking appears to provide reliable values for salivary aldosterone, and the potential application of this technique to the screening of hypertensive patients is discussed.Keywords
This publication has 9 references indexed in Scilit:
- The direct determination of aldosterone in human salivaJournal of Steroid Biochemistry, 1984
- Primary aldosteronism: how hard should we look?BMJ, 1983
- A Simple Solid Phase Enzyme Immunoassay for Aldosterone in Plasma and Saliva1)Experimental and Clinical Endocrinology & Diabetes, 1983
- The changing clinical spectrum of primary aldosteronismAmerican Journal Of Medicine, 1983
- SALIVARY TESTOSTERONE: RELATIONSHIP TO UNBOUND PLASMA TESTOSTERONE IN NORMAL AND HYPERANDROGENIC WOMENClinical Endocrinology, 1982
- A convenient replacement for cyanogen bromide-activated solid phases in immunoradiometric assaysJournal of Immunological Methods, 1982
- Generally applicable 125iodine-based radioimmunoassays for plasma progesteroneSteroids, 1981
- Optimization of a direct radioimmunoassay for plasma aldosteroneJournal of Steroid Biochemistry, 1981
- The Biologic Significance of the Aldosterone Concentration in SalivaPediatric Research, 1979