Iron-Regulated Surface Determinant Protein A Mediates Adhesion of Staphylococcus aureus to Human Corneocyte Envelope Proteins
Open Access
- 1 June 2009
- journal article
- research article
- Published by American Society for Microbiology in Infection and Immunity
- Vol. 77 (6), 2408-2416
- https://doi.org/10.1128/iai.01304-08
Abstract
The ability of Staphylococcus aureus to colonize the human nares is a crucial prerequisite for disease. IsdA is a major S. aureus surface protein that is expressed during human infection and required for nasal colonization and survival on human skin. In this work, we show that IsdA binds to involucrin, loricrin, and cytokeratin K10, proteins that are present in the cornified envelope of human desquamated epithelial cells. To measure the forces and dynamics of the interaction between IsdA and loricrin (the most abundant protein of the cornified envelope), single-molecule force spectroscopy was used, demonstrating high-specificity binding. IsdA acts as a cellular adhesin to the human ligands, promoting whole-cell binding to immobilized proteins, even in the absence of other S. aureus components (as shown by heterologous expression in Lactococcus lactis ). Inhibition experiments revealed the binding of the human ligands to the same IsdA region. This region was mapped to the NEAT domain of IsdA. The NEAT domain also was found to be required for S. aureus whole-cell binding to the ligands as well as to human nasal cells. Thus, IsdA is an important adhesin to human ligands, which predominate in its primary ecological niche.Keywords
This publication has 61 references indexed in Scilit:
- Surface proteins that promote adherence of Staphylococcus aureusto human desquamated nasal epithelial cellsBMC Microbiology, 2009
- IsdA ProtectsStaphylococcus aureusagainst the Bactericidal Protease Activity of ApolactoferrinInfection and Immunity, 2008
- Key Role for Clumping Factor B in Staphylococcus aureus Nasal Colonization of HumansPLoS Medicine, 2008
- Single-Molecule Force Spectroscopy of Mycobacterial Adhesin-Adhesin InteractionsJournal of Bacteriology, 2007
- The Surface Protein Srr-1 of Streptococcus agalactiae Binds Human Keratin 4 and Promotes Adherence to Epithelial HEp-2 CellsInfection and Immunity, 2007
- Characterization of the Heme Binding Properties of Staphylococcus aureus IsdABiochemistry, 2006
- Nanoscale mapping and functional analysis of individual adhesins on living bacteriaNature Methods, 2005
- Staphylococcus aureus Nasal Carriage and Infection in Patients on Continuous Ambulatory Peritoneal DialysisNew England Journal of Medicine, 1990
- Staphylococcus aureusNasal Carriage and Infection in Patients on HemodialysisNew England Journal of Medicine, 1986
- Covalent attachment of ω-hydroxyacid derivatives to epidermal macromolecules: A preliminary characterizationBiochemical and Biophysical Research Communications, 1986