Modulation of Isoflavonoid Composition of Rhizopus oryzae Elicited Soybean (Glycine max) Seedlings by Light and Wounding
- 28 August 2013
- journal article
- research article
- Published by American Chemical Society (ACS) in Journal of Agricultural and Food Chemistry
- Vol. 61 (36), 8657-8667
- https://doi.org/10.1021/jf4020203
Abstract
The isoflavonoid profile of soybean was altered in different ways by stimulation of defense response upon germination. The combination of simultaneous germination and induction by Rhizopus oryzae increased the total isoflavonoid content of soybeans over 2-fold. Pterocarpans became the predominant isoflavonoids, up to 50% (w/w) of total isoflavonoids. To modulate both isoflavonoid content and composition further, the treatment was extended with wounding or light stimuli. The total isoflavonoid content could be increased over 3-fold compared to untreated beans by growing fungus-elicited soybean seedlings in light, whereas wounding was less effective. Interestingly, light altered the composition of prenylated pterocarpans by mediating the position of prenylation. The 2-prenylated pterocarpan level increased 2-fold, whereas that of 4-prenylated pterocarpan remained similar. Taken together, fungus was the most effective elicitor to alter the isoflavonoid content and composition of soybean seedlings, the impact of which can be further enhanced and mediated by additional stimuli, particularly light.Keywords
This publication has 39 references indexed in Scilit:
- Prenylated isoflavonoids from plants as selective estrogen receptor modulators (phytoSERMs)Food & Function, 2012
- Increasing Soy Isoflavonoid Content and Diversity by Simultaneous Malting and Challenging by a Fungus to Modulate EstrogenicityJournal of Agricultural and Food Chemistry, 2011
- Phytoalexin-Enriched Functional FoodsJournal of Agricultural and Food Chemistry, 2009
- Isoflavonoids of the LeguminosaeNatural Product Reports, 2007
- Analysis of phenolic compounds and isoflavones in soybean seeds (Glycine max (L.) Merill) and sprouts grown under different conditionsZeitschrift für Lebensmittel-Untersuchung und Forschung, 2005
- Dietary Isoflavones: Biological Effects and Relevance to Human HealthJournal of Nutrition, 1999
- Phytoestrogens: the biochemistry, physiology, and implications for human health of soy isoflavonesThe American Journal of Clinical Nutrition, 1998
- Isoflavones and their conjugates in soy foods: extraction conditions and analysis by HPLC-mass spectrometryJournal of Agricultural and Food Chemistry, 1994
- A simplified HPLC method for the determination of phytoestrogens in soybean and its processed productsJournal of Agricultural and Food Chemistry, 1990
- Soybean isoflavones. Characterization, determination, and antifungal activityJournal of Agricultural and Food Chemistry, 1974