Process considerations for the asymmetric synthesis of chiral amines using transaminases
- 14 April 2011
- journal article
- review article
- Published by Wiley in Biotechnology & Bioengineering
- Vol. 108 (7), 1479-1493
- https://doi.org/10.1002/bit.23154
Abstract
Biocatalytic transamination is being established as key tool for the production of chiral amine pharmaceuticals and precursors due to its excellent enantioselectivity as well as green credentials. Recent examples demonstrate the potential for developing economically competitive processes using a combination of modern biotechnological tools for improving the biocatalyst alongside using process engineering and integrated separation techniques for improving productivities. However, many challenges remain in order for the technology to be more widely applicable, such as technologies for obtaining high yields and productivities when the equilibrium of the desired reaction is unfavorable. This review summarizes both the process challenges and the strategies used to overcome them, and endeavors to describe these and explain their applicability based on physiochemical principles. This article also points to the interaction between the solutions and the need for a process development strategy based on fundamental principles. Biotechnol. Bioeng. 2011; 108:1479–1493.Keywords
This publication has 80 references indexed in Scilit:
- Exploring challenges in rational enzyme design by simulating the catalysis in artificial kemp eliminaseProceedings of the National Academy of Sciences of the United States of America, 2010
- Biocatalytic Asymmetric Synthesis of Chiral Amines from Ketones Applied to Sitagliptin ManufactureScience, 2010
- Chiral Amine Synthesis – Recent Developments and Trends for Enamide Reduction, Reductive Amination, and Imine ReductionAdvanced Synthesis & Catalysis, 2010
- Directed enzyme evolution: climbing fitness peaks one amino acid at a timeCurrent Opinion in Chemical Biology, 2009
- Whole-cell bioreduction of aromatic α-keto esters using Candida tenuis xylose reductase and Candida boidinii formate dehydrogenase co-expressed in Escherichia coliMicrobial Cell Factories, 2008
- Group Contribution Method for Thermodynamic Analysis of Complex Metabolic NetworksBiophysical Journal, 2008
- Enzyme Immobilization: The Quest for Optimum PerformanceAdvanced Synthesis & Catalysis, 2007
- Covalent immobilization of ω-transaminase from Vibrio fluvialis JS17 on chitosan beadsProcess Biochemistry, 2007
- Cloning and Characterization of a Novel β-Transaminase fromMesorhizobiumsp. Strain LUK: a New Biocatalyst for the Synthesis of Enantiomerically Pure β-Amino AcidsApplied and Environmental Microbiology, 2007
- Industrial Methods for the Production of Optically Active IntermediatesAngewandte Chemie-International Edition, 2004