Kinetics of pump currents generated by the Na+,K+‐ATPase
- 16 November 1987
- journal article
- Published by Wiley in FEBS Letters
- Vol. 224 (1), 83-88
- https://doi.org/10.1016/0014-5793(87)80427-1
Abstract
Purified Na+,K+-ATPase from pig kidney was attached to black lipid membranes. Pump currents of the enzyme could be measured with a time resolution of approx. 1 ms by releasing ATP from caged ATP with a UV laser flash. Analysis of the transient currents shows that a slow non-electrogenic step is followed by an electrogenic transition with a rate constant of 100 s−1 (22°C). The exponential components found in the transient currents are compared to transitions in the Albers-Post schemeKeywords
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