The Manganese Site of the Photosynthetic Water-Splitting Enzyme
- 10 February 1989
- journal article
- Published by American Association for the Advancement of Science (AAAS) in Science
- Vol. 243 (4892), 789-791
- https://doi.org/10.1126/science.2916124
Abstract
As the originator of the oxygen in our atmosphere, the photosynthetic water-splitting enzyme of chloroplasts is vital for aerobic life on the earth. It has a manganese cluster at its active site, but it is poorly understood at the molecular level. Polarized synchrotron radiation was used to examine the x-ray absorption of manganese in oriented chloroplasts. The manganese site, in the "resting" (S1) state, is an asymmetric cluster, which probably contains four manganese atoms, with interatomic separations of 2.7 and 3.3 angstroms; the vector formed by the 3.3-angstrom manganese pair is oriented perpendicular to the membrane plane. Comparisons with model compounds suggest that the cluster contains bridging oxide or hydroxide ligands connecting the manganese atoms, perhaps with carboxylate bridges connecting the 3.3-angstrom manganese pair.Keywords
This publication has 25 references indexed in Scilit:
- Characterization of the manganese oxygen-evolving complex and the iron-quinone acceptor complex in photosystem II from a thermophilic cyanobacterium by electron paramagnetic resonance and x-ray absorption spectroscopyBiochemistry, 1988
- Optical-difference spectra of the S-state transitions in the photosynthetic oxygen-evolving complexBiochimica et Biophysica Acta (BBA) - Bioenergetics, 1987
- Water binding to the oxygen-evolving system of chloroplasts; effects of isotope substitution on the S2 state EPR spectrumBiochimica et Biophysica Acta (BBA) - Bioenergetics, 1987
- Oxygen from water is coordinated to manganese in the S2 state of photosystem IIFEBS Letters, 1986
- Polarized x-ray absorption edge spectroscopy of single-crystal copper(II) complexesJournal of the American Chemical Society, 1985
- Orientation of EPR signals arising from components in Photosystem II membranesBiochimica et Biophysica Acta (BBA) - Bioenergetics, 1985
- Synthesis of a Tetranuclear Manganese(IV) Cluster with Adamantane Skeleton: [(C6H15N3)4Mn4O6]4+Angewandte Chemie-International Edition, 1983
- Orientation of membrane‐bound cytochromes in chloroplasts, detected by low‐temperature EPR spectroscopyFEBS Letters, 1982
- Theory of extended x-ray absorption fine structure: Single and multiple scattering formalismsThe Journal of Chemical Physics, 1982
- Extended X-ray absorption fine structure determination of iron nitrogen distances in haemoglobinNature, 1978