2D luminescence imaging of pH in vivo
Open Access
- 24 January 2011
- journal article
- research article
- Published by Proceedings of the National Academy of Sciences in Proceedings of the National Academy of Sciences of the United States of America
- Vol. 108 (6), 2432-2437
- https://doi.org/10.1073/pnas.1006945108
Abstract
Luminescence imaging of biological parameters is an emerging field in biomedical sciences. Tools to study 2D pH distribution are needed to gain new insights into complex disease processes, such as wound healing and tumor metabolism. In recent years, luminescence-based methods for pH measurement have been developed. However, for in vivo applications, especially for studies on humans, biocompatibility and reliability under varying conditions have to be ensured. Here, we present a referenced luminescent sensor for 2D high-resolution imaging of pH in vivo. The ratiometric sensing scheme is based on time-domain luminescence imaging of FITC and ruthenium(II)tris-(4,7-diphenyl-1,10-phenanthroline). To create a biocompatible 2D sensor, these dyes were bound to or incorporated into microparticles (aminocellulose and polyacrylonitrile), and particles were immobilized in polyurethane hydrogel on transparent foils. We show sensor precision and validity by conducting in vitro and in vivo experiments, and we show the versatility in imaging pH during physiological and chronic cutaneous wound healing in humans. Implementation of this technique may open vistas in wound healing, tumor biology, and other biomedical fields.Keywords
This publication has 47 references indexed in Scilit:
- Wound healing in the 21st centuryJournal of the American Academy of Dermatology, 2010
- Disease-causing Mutations in the Cystic Fibrosis Transmembrane Conductance Regulator Determine the Functional Responses of Alveolar MacrophagesOnline Journal of Public Health Informatics, 2009
- A mathematical model of ischemic cutaneous woundsProceedings of the National Academy of Sciences of the United States of America, 2009
- A dual-emissive-materials design concept enables tumour hypoxia imagingNature Materials, 2009
- In vivo fluorescence imaging with high-resolution microlensesNature Methods, 2009
- A tissue-scale gradient of hydrogen peroxide mediates rapid wound detection in zebrafishNature, 2009
- Wound angiogenesis as a function of tissue oxygen tension: A mathematical modelProceedings of the National Academy of Sciences of the United States of America, 2008
- Expanded dynamic range of fluorescent indicators for Ca 2+ by circularly permuted yellow fluorescent proteinsProceedings of the National Academy of Sciences of the United States of America, 2004
- Circularly permuted green fluorescent proteins engineered to sense Ca 2+Proceedings of the National Academy of Sciences of the United States of America, 2001
- STATISTICAL METHODS FOR ASSESSING AGREEMENT BETWEEN TWO METHODS OF CLINICAL MEASUREMENTThe Lancet, 1986