RNA Pol II Accumulates at Promoters of Growth Genes During Developmental Arrest
- 3 April 2009
- journal article
- other
- Published by American Association for the Advancement of Science (AAAS) in Science
- Vol. 324 (5923), 92-94
- https://doi.org/10.1126/science.1169628
Abstract
When Caenorhabditis elegans larvae hatch from the egg case in the absence of food, their development is arrested (L1 arrest), and they show increased stress resistance until food becomes available. To study nutritional control of larval development, we analyzed growth and gene expression profiles during L1 arrest and recovery. Larvae that were fed responded relatively slowly to starvation compared with the rapid response of arrested larvae to feeding. Chromatin immunoprecipitation of RNA polymerase II (Pol II) followed by deep sequencing showed that during L1 arrest, Pol II continued transcribing starvation-response genes, but the enzyme accumulated on the promoters of growth and development genes. In response to feeding, promoter accumulation decreased, and elongation and messenger RNA levels increased. Therefore, accumulation of Pol II at promoters anticipates nutritionally controlled gene expression during C. elegans development.Keywords
This publication has 20 references indexed in Scilit:
- The Genomic Distribution and Function of Histone Variant HTZ-1 during C. elegans EmbryogenesisPLoS Genetics, 2008
- NELF-mediated stalling of Pol II can enhance gene expression by blocking promoter-proximal nucleosome assemblyGenes & Development, 2008
- NELF and GAGA Factor Are Linked to Promoter-Proximal Pausing at Many Genes in DrosophilaMolecular and Cellular Biology, 2008
- RNA polymerase stalling at developmental control genes in the Drosophila melanogaster embryoNature Genetics, 2007
- RNA polymerase is poised for activation across the genomeNature Genetics, 2007
- A Chromatin Landmark and Transcription Initiation at Most Promoters in Human CellsCell, 2007
- High-Resolution Profiling of Histone Methylations in the Human GenomeCell, 2007
- A Mediator subunit, MDT-15, integrates regulation of fatty acid metabolism by NHR-49-dependent and -independent pathways inC. elegansGenes & Development, 2006
- DAF-16/FOXO Regulates Transcription of cki-1/Cip/Kip and Repression of lin-4 during C. elegans L1 ArrestCurrent Biology, 2006
- Nuclear Hormone Receptor NHR-49 Controls Fat Consumption and Fatty Acid Composition in C. elegansPLoS Biology, 2005