NAP protects against cyanide-related microtubule destruction
- 24 June 2009
- journal article
- Published by Springer Science and Business Media LLC in Journal of Neural Transmission
- Vol. 116 (11), 1411-1416
- https://doi.org/10.1007/s00702-009-0252-7
Abstract
The peptide NAP (NAPVSIPQ) was shown to protect neurons against a wide variety of insults. Particularly, NAP was shown to be neuroprotective in vitro against cyanide in hippocampal cultures and against oxygen-glucose deprivation in hippocampal and cortical neuronal cultures. Cyanide causes energy depletion in the cell and destroys the cytoskeleton, and NAP has been shown before to protect the microtubule cytoskeleton. The current study explored the effect of NAP on cyanide-induced microtubule destruction in cerebral cortical cultures. Sodium cyanide (6.8 mM) reduced the number of neurons containing intact microtubules as identified by bIII-tubulin immunostaining. When sodium cyanide was added together with NAP (10−14–10−12 M), complete protection was observed. Although the primary site of action of cyanide is considered to be the mitochondria, the current results involve microtubule destruction by cyanide toxicity that is completely reversed by NAP treatment.Keywords
This publication has 53 references indexed in Scilit:
- NAP protects memory, increases soluble tau and reduces tau hyperphosphorylation in a tauopathy modelNeurobiology of Disease, 2009
- Frontotemporal dementia and related disorders: Deciphering the enigmaAnnals of Neurology, 2008
- Interaction of nitric oxide with a functional model of cytochrome c oxidaseProceedings of the National Academy of Sciences of the United States of America, 2008
- Resistance to kynurenic acid of the NMDA receptor-dependent toxicity of 3-nitropropionic acid and cyanide in cerebellar granule neuronsBrain Research, 2008
- A Neuronal Microtubule-Interacting Agent, NAPVSIPQ, Reduces Tau Pathology and Enhances Cognitive Function in a Mouse Model of Alzheimer's DiseaseJournal of Pharmacology and Experimental Therapeutics, 2008
- Activity-Dependent Neuroprotective Protein Snippet NAP Reduces Tau Hyperphosphorylation and Enhances Learning in a Novel Transgenic Mouse ModelThe Journal of pharmacology and experimental therapeutics, 2007
- The peptide NAP promotes neuronal growth and differentiation through extracellular signal‐regulated protein kinase and Akt pathways, and protects neurons co‐cultured with astrocytes damaged by ethanolJournal of Neurochemistry, 2007
- Brain deficits associated with fetal alcohol exposure may be protected, in part, by peptides derived from activity-dependent neurotrophic factor and activity-dependent neuroprotective proteinBrain Research Reviews, 2006
- Mitochondrial organization and motility probed by two-photon microscopy in cultured mouse brainstem neuronsExperimental Cell Research, 2004
- Neuropathological stageing of Alzheimer-related changesActa Neuropathologica, 1991