A genetic linkage map for the domesticated silkworm,Bombyx mori, based on restriction fragment length polymorphisms
- 1 October 1995
- journal article
- research article
- Published by Hindawi Limited in Genetics Research
- Vol. 66 (2), 109-126
- https://doi.org/10.1017/s0016672300034467
Abstract
Summary: We present data for the initial construction of a molecular linkage map for the domesticated silkworm,Bombyx mori, based on 52 progeny from an F2 cross from a pair mating of inbred strains p50 and C108, using restriction fragment length polymorphisms (RFLPs). The map contains 15 characterized single copy sequences, 36 anonymous sequences derived from a follicular cDNA library, and 10 loci corresponding to a low copy number retrotransposon, mag. The 15 linkage groups and 8 ungrouped loci account for 23 of the 28 chromosomes and span a total recombination length of 413 cM; 10 linkage groups were correlated with established classic genetic maps. Scoring data from Southern blots were analysed using two Pascal programs written specifically to analyse linkage data in Lepidoptera, where females are the heterogametic sex and have achiasmatic meiosis (no crossing-over). These first examine evidence for linkage by calculating the maximum lod score under the hypothesis that the two loci are linked over the likelihood under the hypothesis that the two loci assort independently, and then determine multilocus linkage maps for groups of putatively syntenic loci by calculating the maximum likelihood estimate of the recombination fractions and the log likelihood using the EM algorithm for a specified order of loci along the chromosome. In addition, the possibility of spurious linkage was exhaustively tested by searching for genotypes forbidden by the absence of crossing-over in one sex.Keywords
This publication has 68 references indexed in Scilit:
- Linkage map of random amplified polymorphic DNAs (RAPDs) in the silkworm,Bombyx moriGenetics Research, 1995
- Primary structure of the silk fibroin light chain determined by cDNA sequencing and peptide analysisJournal of Molecular Biology, 1989
- MAPMAKER: An interactive computer package for constructing primary genetic linkage maps of experimental and natural populationsGenomics, 1987
- Manifestation of characters in the "Giant egg" mutant of Bombyx mori (Lepidoptera: Bombycidae). 1. Characteristics of the Ge egg.Japanese Journal of Applied Entomology and Zoology, 1987
- Transposable genetic element found in the 5′-flanking region of the fibroin H-chain gene in a genomic clone from the silkworm Bombyx moriJournal of Molecular Biology, 1986
- A highly reiterated family of transcribed oligo(A)-terminated, interspersed DNA elements in the genome of Bombyx moriJournal of Molecular Biology, 1986
- GENETICAL AND BIOCHEMICAL STUDIES OF PTERIDINE GRANULE MEMBRANE IN LARVAL HYPODERMAL CELLS OF THE SILKWORMThe Japanese Journal of Genetics, 1976
- GENETICAL AND BIOCHEMICAL STUDIES OF PTERIDINE GRANULE MEMBRANE IN LARVAL HYPODERMAL CELLS OF THE SILKWORMThe Japanese Journal of Genetics, 1976
- Polyploidization of the silk gland of Bombyx moriJournal of Molecular Biology, 1974
- No Crossing Over in the Female of the Silkworm MothThe American Naturalist, 1915