Factors affecting DTPA‐extractable Zn, Fe, Mn, and Cu from soils

Abstract

Tests were made to determine the effects of grinding, type of extraction vessel, type of shaker, speed of shaking, time of shaking, time of filtering, soil to solution ratio and other variables on DTPA[diethylenetriaminopentaacetic acid]-extractable Zn, Fe, Mn and Cu from soils. Time of grinding, force of grinding and the quantity of soil being ground greatly affected the amount of extractable Fe. At the lower grinding force, the quantity of soil being ground only slightly affected extractable Fe, but at the higher grinding force, more Fe was extracted from the smaller sized samples especially at the longer grinding period. Extractable Zn was also increased by longer grinding time and greater grinding force, but increases were much less than increases for Fe. Increasing grinding time tended to increase extractable Mn. The effects of grinding on Cu was inconclusive. Increasing the ratio of extractant to soil increased the amount of extractable Fe from soils and tended to increase Zn, Mn and Cu but to a lesser extent. Both shaker speed and type of extracting vessel affected the extractability of all nutrients except Cu. Greatest differences between extracting vessels occurred at the lowest shaker speed, while these differences were smaller or disappeared at the higher shaker speeds. The more thorough the mixing of soil and extracting solution, the higher were the levels of extractable Fe and Mn. A reciprocal shaker extracted more Fe and Mn from soils than a rotary shaker. The rate of dissolution of all 4 nutrients by DTPA was greatest during the first 5 min of extraction. There were large and significant correlation coefficients between levels of nutrients extracted after 15 or 30 min of shaking and those extracted after 120 min. The levels of micronutrients extracted under 1 set of conditions can be related to levels extracted under other conditions by use of a simple linear regression equation for each nutrient. The importance of standardizing the methods of preparation and extraction of soils used in the DTPA micronutrient soil test is demonstrated. A standard method for soil grinding and extraction is proposed for DTPA soil test.