Altered localization of CXCL13 expressing cells in mice deficient in Pactolus following an inflammatory stimulus
- 1 October 2006
- journal article
- Published by Wiley in Immunology
- Vol. 119 (2), 212-223
- https://doi.org/10.1111/j.1365-2567.2006.02426.x
Abstract
The mouse Pactolus gene is an evolutionary paralogue to the CD18/beta2 integrin subunit and is preferentially expressed by neutrophils. When first identified, it was assumed Pactolus would function as an adhesion receptor similar to other beta integrin subunits. The analysis of mice genetically deficient in Pactolus, however, did not define any lesion in neutrophil migration, adhesion or phagocytosis. Therefore a wider analysis of the Pactolus deficiency was initiated using transcriptional profiling during an inflammatory insult. This screen identified a single transcript, CXCL13, that was elevated in cells from a peritoneal lavage of the wild type animal compared to the Pactolus-deficient animal. Our analyses confirmed resident macrophages as being responsible for the chemokine using intracellular CXCL13 staining and additional cell markers to phenotypically characterize such cells. The resident CXCL13-expressing cells (which do not express Pactolus) are functionally distinct from the macrophages recruited into the peritoneal cavity following the inflammatory stimulation since the recruited macrophages do not express detectable levels of the chemokine. The numbers and expression patterns of these resident CXCL13-expressing cells do not vary in naïve animals of wild type or Pactolus-deficient origin. Additionally, Pactolus-deficient neutrophils do not preferentially kill (compared to wild type) CXC13-expressing macrophages. These data suggest that during an inflammatory response, Pactolus may help retain CXCL13-expressing cells within the peritoneal environment.Keywords
This publication has 28 references indexed in Scilit:
- B-Cells in Ocular Adnexal Lymphoproliferative Lesions Express B-cell attracting Chemokine 1 (CXCL13)American Journal of Ophthalmology, 2005
- BCA-1/CXCL13 expression is associated with CXCR5-positive B-cell cluster formation in acute renal transplant rejectionKidney International, 2005
- Phagocytosis of Microbes: Complexity in ActionAnnual Review of Immunology, 2002
- CXCL13 Is Required for B1 Cell Homing, Natural Antibody Production, and Body Cavity ImmunityImmunity, 2002
- Functional Characterization of Pactolus, a β-Integrin-like Protein Preferentially Expressed by NeutrophilsPublished by Elsevier BV ,2001
- Identification of Pactolus, an Integrin β Subunit-like Cell-surface Protein Preferentially Expressed by Cells of the Bone MarrowPublished by Elsevier BV ,1998
- Neutrophil Emigration in the Skin, Lungs, and Peritoneum: Different Requirements for CD11/CD18 Revealed by CD18-deficient MiceThe Journal of Experimental Medicine, 1997
- The A-domain of beta 2 integrin CR3 (CD11b/CD18) is a receptor for the hookworm-derived neutrophil adhesion inhibitor NIF.The Journal of cell biology, 1994
- A novel divalent cation-binding site in the a domain of the ?2 integrin CR3 (CD11b/CD18) is essential for ligand bindingCell, 1993
- Isolation of biologically active ribonucleic acid from sources enriched in ribonucleaseBiochemistry, 1979