Effect of Ultrasound on DNA Polymerase Reactions: Monitoring on a 27-MHz Quartz Crystal Microbalance
- 2 February 2006
- journal article
- Published by American Chemical Society (ACS) in Biomacromolecules
- Vol. 7 (3), 682-685
- https://doi.org/10.1021/bm050738e
Abstract
Effects of ultrasound irradiation on DNA polymerase (Klenow fragment, KF) reactions were studied on the template/primer DNA-immobilized quartz crystal microbalance (QCM). Under ultrasound irradiation, binding of KF to the DNA was suppressed due to the decrease of the binding rate constant (k1) and the increase of the dissociation rate constant (k-1). The catalytic elongation rate (kcat) was increased, but the stability of the KF/DNA/monomer ternary complex (Km) was decreased by the ultrasound irradiation. Ultrasound effects are discussed in correlation with the conformation changes of domain structures in KF.Keywords
This publication has 23 references indexed in Scilit:
- Kinetic Studies of Site-Directed Mutational Isomalto-dextranase-Catalyzed Hydrolytic Reactions on a 27 MHz Quartz-Crystal MicrobalanceBiochemistry, 2005
- The Hydration of Globular Proteins as Derived from Volume and Compressibility Measurements: Cross Correlating Thermodynamic and Structural DataJournal of Molecular Biology, 1996
- Molecular Recognition by Self-Assembled Monolayers of Cavitand ReceptorsScience, 1994
- Movable lobes and flexible loops in proteins Structural deformations that control biochemical activityFEBS Letters, 1993
- Kinetic mechanism of DNA polymerase I(Klenow fragment): identification of a second conformational change and evaluation of the internal equilibrium constantBiochemistry, 1991
- Cell Proliferation in CarcinogenesisScience, 1990
- Low-frequency ultrasound absorption in aqueous solutions of hemoglobin, myoglobin, and bovine serum albumin: The role of structure and pHThe Journal of the Acoustical Society of America, 1988
- Ultrasonic relaxation study of aqueous solutions of poly(acrylic acid)Macromolecules, 1985
- Elementary steps in the DNA polymerase I reaction pathwayBiochemistry, 1983
- Verwendung von Schwingquarzen zur W gung d nner Schichten und zur Mikrow gungThe European Physical Journal A, 1959