Silver as a Probe of Pore-Forming Residues in a Potassium Channel
- 14 April 1995
- journal article
- other
- Published by American Association for the Advancement of Science (AAAS) in Science
- Vol. 268 (5208), 304-307
- https://doi.org/10.1126/science.7716526
Abstract
In voltage-dependent potassium channels, the molecular determinants of ion selectivity are found in the P (pore) region, a stretch of 21 contiguous residues. Cysteine was introduced at each P region position in a Shaker potassium channel. Residues projecting side chains into the pore were identified by means of channel inhibition by a sulfhydryl-reactive potassium ion analog, silver ion. The pattern of silver ion reactivity contradicts a beta barrel architecture of potassium channel pores.Keywords
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