Molecular Characterization of the SHV-11 β-Lactamase of Shigella dysenteriae
- 1 August 1999
- journal article
- research article
- Published by American Society for Microbiology in Antimicrobial Agents and Chemotherapy
- Vol. 43 (8), 2081-2083
- https://doi.org/10.1128/aac.43.8.2081
Abstract
A β-lactamase with an M r of 29,000 and a pI of 7.6 was partially purified from a clinical isolate of Shigella dysenteriae . The bla gene encoded the SHV-11 enzyme carrying the substitution Leu→Gln at position 35 and was linked to a strong promoter. This variant, unlike the prototype SHV-1 enzyme, hydrolyzed oxacillin, cloxacillin, and oxyiminocephalosporins such as cefotaxime.Keywords
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