Fluorescence Polarization Based Nucleic Acid Testing for Rapid and Cost‐Effective Diagnosis of Infectious Disease
- 30 September 2015
- journal article
- research article
- Published by Wiley in Chemistry – A European Journal
- Vol. 21 (46), 16359-16363
- https://doi.org/10.1002/chem.201502934
Abstract
A new nucleic acid detection method was developed for a rapid and cost‐effective diagnosis of infectious disease. This approach relies on the three unique elements: 1) detection probes that regulate DNA polymerase activity in response to the complementary target DNA; 2) universal reporters conjugated with a single fluorophore; and 3) fluorescence polarization (FP) detection. As a proof‐of‐concept, the assay was used to detect and sub‐type Salmonella bacteria with sensitivities down to a single bacterium in less than three hours.Keywords
Funding Information
- NIH (R01L113156, R01EB004626, R01EB010011, HHSN268201000044C, R33100023, W81XWH-14-1-0279)
- National Research Foundation of Korea
- Ministry of Education (2014R1A6A3A03059728)
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