NMR Analysis of Low-Density Lipoprotein Oxidatively-Modifiedin vztro

Abstract

Human plasma low density lipoprotein has been oxidized at different stages in vitro and analysed by ¹H. ¹³C, and ³¹P NMR spectroscopy and by biochemical methods. Information was obtained on: a) structure mobilities of lipids and on lipid-protein interactions: b) conjugated and oxo-dienes; c) polyunsaturated/ monounsaturated fatty acid chains; d) lysophosphatidylcholine production. The results show that the NMR approach is particularly useful for the assessment of structural modification in oxidized LDL.