Autocrine growth and tumorigenicity of interleukin 2-dependent helper T cells transfected with IL-2 gene.
Open Access
- 1 January 1989
- journal article
- research article
- Published by Rockefeller University Press in The Journal of Experimental Medicine
- Vol. 169 (1), 13-25
- https://doi.org/10.1084/jem.169.1.13
Abstract
We introduced a mouse IL-2 cDNA expression vector into an IL-2-dependent mouse helper T cell line HT-2. Transfected cells secreted substantial amounts of IL-2, to which they themselves responded by proliferating without further requirement for exogenous IL-2. The proliferation was a direct function of the cell density and was inhibitable by antibodies against IL-2 or IL-2-R, indicating the autocrine nature of the proliferation. Those producing higher amounts of IL-2 were found to be tumorigenic when inoculated into nude mice. The latency period of tumor development correlated inversely with the level of IL-2 secreted. Tumor cells proliferated in vitro in an IL-2 autocrine fashion indistinguishable from that of the inoculated cells. We thus provide evidence that the aberrant activation of the IL-2 autocrine circuit can lead T cells to malignant transformation.This publication has 47 references indexed in Scilit:
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