Analysis of meticillin-susceptible and meticillin-resistant biofilm-forming Staphylococcus aureus from catheter infections isolated in a large Italian hospital
- 1 March 2008
- journal article
- Published by Microbiology Society in Journal of Medical Microbiology
- Vol. 57 (3), 364-372
- https://doi.org/10.1099/jmm.0.47621-0
Abstract
Several characteristics were analysed in 37 Staphylococcus aureus isolates from nosocomial catheter infections: the PFGE profile after SmaI digestion of chromosomal DNA, the ability to form a biofilm on a polystyrene surface, antibiotic susceptibility patterns (penicillin, oxacillin, erythromycin, tetracycline, clindamycin, telithromycin, gentamicin, ciprofloxacin, quinupristin/dalfopristin, rifampicin, vancomycin and linezolid), and the presence of genetic determinants of antibiotic resistance and biofilm formation. All strains but three (92 %) were able to grow on a plastic surface as a biofilm. An almost complete association was found between phenotypes and genotypic traits of antibiotic resistance, whilst PFGE profiling showed the highly polyclonal composition of the set of strains under study. Sixteen isolates (43 %) were meticillin-resistant and were subjected to staphylococcal cassette chromosome mec (SCCmec) and cassette chromosome recombinase (ccr) complex type determination by multiplex PCR. Only a subgroup of six strains belonged to the archaic clone PFGE type and bore the SCCmec/ccrAB type I structure. Among the remaining strains some presented small rearrangements of the SCCmec/ccrAB genetic locus, whilst others could barely be traced back to a known structural type. These observations suggest that, at the local level and at a particular site of infection, S. aureus may show great genetic variability and escape the general rule of expansion of the S. aureus pandemic clones.Keywords
This publication has 38 references indexed in Scilit:
- The molecular evolution of methicillin-resistant Staphylococcus aureusClinical Microbiology & Infection, 2007
- Activity of the major staphylococcal autolysin AtlFEMS Microbiology Letters, 2006
- The clinical significance of methicillin-resistant Staphylococcus aureusJournal of Hospital Infection, 2005
- Presence of the ica operon in clinical isolates of Staphylococcus epidermidis and its role in biofilm productionClinical Microbiology & Infection, 2004
- Biofilms: Survival Mechanisms of Clinically Relevant MicroorganismsClinical Microbiology Reviews, 2002
- In catheter infections by Staphylococcus epidermidis the intercellular adhesion (ica) locus is a molecular marker of the virulent slime‐producing strainsJournal of Biomedical Materials Research, 2001
- The evolution of methicillin resistance in Staphylococcus aureus : Similarity of genetic backgrounds in historically early methicillin-susceptible and -resistant isolates and contemporary epidemic clonesProceedings of the National Academy of Sciences of the United States of America, 2001
- Molecular Typing of Methicillin-ResistantStaphylococcus aureusby Pulsed-Field Gel Electrophoresis: Comparison of Results Obtained in a Multilaboratory Effort Using Identical Protocols and MRSA StrainsMicrobial Drug Resistance, 2000
- Similarity of Antibiotic Resistance Patterns and Molecular Typing Properties of Methicillin-ResistantStaphylococcus aureusIsolates Widely Spread in Hospitals in New York City and in a Hospital in Tokyo, JapanMicrobial Drug Resistance, 2000
- Methicillin-Resistant Staphylococcus Aureus and Antimicrobial Use in Belgian HospitalsInfection Control & Hospital Epidemiology, 1999