Role of RNA Structure and Susceptibility to RNase E in Regulation of a Cold Shock mRNA, cspA mRNA
- 15 June 2007
- journal article
- Published by American Society for Microbiology in Journal of Bacteriology
- Vol. 189 (12), 4353-4358
- https://doi.org/10.1128/jb.00193-07
Abstract
Degradation of the cspA mRNA in vivo is very rapid at temperatures greater than 30°C and is moderately dependent on RNase E. Investigations in vitro show that degradosomes prepared from normal or cold-shocked cultures cleave the cspA mRNA preferentially at a single site in vitro between two stem-loops ∼24 residues 3′ to the termination codon and ∼31 residues from the 3′ end. The site of cleavage is independent of the temperature and largely independent of the phosphorylation status of the 5′ end of cspA mRNA. A 5′ stem-loop, potential occlusion of the initiation and termination codons, temperature-dependent translational efficiency, and the position of the RNase E cleavage site can explain the differential stability of the cspA mRNA.Keywords
This publication has 35 references indexed in Scilit:
- The Cold Box Stem-loop Proximal to the 5′-End of theEscherichia coli cspA Gene Stabilizes Its mRNA at Low TemperatureJournal of Biological Chemistry, 2002
- Stabilization of Circular rpsT mRNA Demonstrates the 5′-End Dependence of RNase E Action in VivoJournal of Biological Chemistry, 2000
- Cold-shock response and cold-shock proteinsCurrent Opinion in Microbiology, 1999
- In the absence of translation, RNase E can bypass 5′ mRNA stabilizers in Escherichia coliJournal of Molecular Biology, 1998
- Reconstitution of the degradation of the mRNA for ribosomal protein S20 with purified enzymesJournal of Molecular Biology, 1998
- Promoter‐independent cold‐shock induction of cspA and its derepression at 37°C by mRNA stabilizationMolecular Microbiology, 1997
- RNAdraw: an integrated program for RNA secondary structure calculation and analysis under 32-bit Microsoft WindowsBioinformatics, 1996
- Post‐transcriptional regulation of CspA expression in Escherichia coliMolecular Microbiology, 1996
- Differential mRNA stability of the cspA gene in the cold‐shock response of Escherichia coliMolecular Microbiology, 1996
- A 5'-terminal stem-loop structure can stabilize mRNA in Escherichia coli.Genes & Development, 1992