Purification and characterization of transcribed RNAs using gel filtration chromatography
- 13 December 2007
- journal article
- Published by Springer Science and Business Media LLC in Nature Protocols
- Vol. 2 (12), 3270-3277
- https://doi.org/10.1038/nprot.2007.480
Abstract
RNA synthesis using in vitro transcription by phage T7 RNA polymerase allows preparation of milligram quantities of RNA for biochemical, biophysical and structural investigations. Previous purification approaches relied on gel electrophoretic or gravity-flow chromatography methods. We present here a protocol for the in vitro transcription of RNAs and subsequent purification using fast-performance liquid chromatography. This protocol greatly facilitates production of RNA in a single day from transcription to purification.This publication has 29 references indexed in Scilit:
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