AN EVALUATION OF A CHEMICAL METHOD FOR THE ESTIMATION OF OESTRIOL, OESTRONE AND OESTRADIOL-17β IN HUMAN URINE
- 1 November 1957
- journal article
- research article
- Published by Bioscientifica in Journal of Endocrinology
- Vol. 16 (1), 41-48
- https://doi.org/10.1677/joe.0.0160041
Abstract
SUMMARY: The method described by Brown [1955] for the estimation of oestrone, oestradiol-17β and oestriol in urine has been critically investigated. 1. All checks on specificity so far carried out have confirmed that the method is specific for these oestrogens. 2. The method gives recoveries of 70–95%. 3. The precision of the method has been calculated for oestrogen levels between 0·0 and 40·0 μg/24 hr. From the figures obtained the fiducial ranges and percentage errors of determinations and the limits of sensitivity of the method have been calculated. 4. A number of substances which may occur in urine and which interfere in the method are listed.Keywords
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