Alteration of the Protein Kinase Binding Domain Enhances Function of the Saccharomyces cerevisiae Molecular Chaperone Cdc37
Open Access
- 1 August 2007
- journal article
- research article
- Published by American Society for Microbiology in Eukaryotic Cell
- Vol. 6 (8), 1363-1372
- https://doi.org/10.1128/ec.00165-07
Abstract
Cdc37 is a molecular chaperone that has a general function in the biogenesis of protein kinases. We identified mutations within the putative “protein kinase binding domain” of Cdc37 that alleviate the conditional growth defect of a strain containing a temperature-sensitive allele, tpk2 (Ts), of the cyclic AMP-dependent protein kinase (PKA). These dominant mutations alleviate the temperature-sensitive growth defect by elevating PKA activity, as judged by their effects on PKA-regulated processes, localization and phosphorylation of the PKA effector Msn2, as well as in vitro PKA activity. Although the tpk2 (Ts) growth defect is also alleviated by Cdc37 overproduction, the CDC37 dominant mutants contain wild-type Cdc37 protein levels. In addition, Saccharomyces cerevisiae Ste11 protein kinase has an elevated physical interaction with the altered Cdc37 protein. These results implicate specific amino-terminal residues in the interaction between Cdc37 and client protein kinases and provide further genetic and biochemical support for a model in which Cdc37 functions as a molecular chaperone for protein kinases.Keywords
This publication has 59 references indexed in Scilit:
- Cdc37 has distinct roles in protein kinase quality control that protect nascent chains from degradation and promote posttranslational maturationThe Journal of cell biology, 2007
- Cdc37 maintains cellular viability in Schizosaccharomyces pombe independently of interactions with heat‐shock protein 90The FEBS Journal, 2005
- PP2A Phosphatase Activity Is Required for Stress and Tor Kinase Regulation of Yeast Stress Response Factor Msn2pEukaryotic Cell, 2004
- Stimulation of the weak ATPase activity of human Hsp90 by a client protein 1 1Edited by G. von HeijneJournal of Molecular Biology, 2002
- A Morphogenesis Checkpoint Monitors the Actin Cytoskeleton in YeastThe Journal of cell biology, 1998
- Cdc37 is a molecular chaperone with specific functions in signal transduction.Genes & Development, 1997
- The Yeast CDC37 Gene Interacts with MPS1 and Is Required for Proper Execution of Spindle Pole Body DuplicationThe Journal of cell biology, 1997
- TheKIN28Gene is Required both for RNA Polymerase II Mediated Transcription and Phosphorylation of the Rpb1p CTDJournal of Molecular Biology, 1995
- Isolation, crystallization and preliminary crystallographic data of aspartate aminotransferase from chicken heart mitochondriaJournal of Molecular Biology, 1977
- A genetic analysis of the att-int-xis region of coliphage lambdaJournal of Molecular Biology, 1977