Design of Gold Nanoparticle‐Based Colorimetric Biosensing Assays
Top Cited Papers
- 6 October 2008
- journal article
- review article
- Published by Wiley in ChemBioChem
- Vol. 9 (15), 2363-2371
- https://doi.org/10.1002/cbic.200800282
Abstract
Gold nanoparticle (AuNP)‐based colorimetric biosensing assays have recently attracted considerable attention in diagnostic applications due to their simplicity and versatility. This Minireview summarizes recent advances in this field and attempts to provide general guidance on how to design such assays. The key to the AuNP‐based colorimetric sensing platform is the control of colloidal AuNP dispersion and aggregation stages by using biological processes (or analytes) of interest. The ability to balance interparticle attractive and repulsive forces, which determine whether AuNPs are stabilized or aggregated and, consequently, the color of the solution, is central in the design of such systems. AuNP aggregation in these assays can be induced by an “interparticle‐crosslinking” mechanism in which the enthalpic benefits of interparticle bonding formation overcome interparticle repulsive forces. Alternatively, AuNP aggregation can be guided by the controlled loss of colloidal stability in a “noncrosslinking‐aggregation” mechanism. In this case, as a consequence of changes in surface properties, the van der Waals attractive forces overcome interparticle repulsive forces. Using representative examples we illustrate the general strategies that are commonly used to control AuNP aggregation and dispersion in AuNP‐based colorimetric assays. Understanding the factors that play important roles in such systems will not only provide guidance in designing AuNP‐based colorimetric assays, but also facilitate research that exploits these principles in such areas as nanoassembly, biosciences and colloid and polymer sciences.This publication has 139 references indexed in Scilit:
- Gold Nanoparticle Sensor for Homocysteine Thiolactone-Induced Protein ModificationLangmuir, 2008
- Synthesis and electrochemical applications of gold nanoparticlesAnalytica Chimica Acta, 2007
- Screening the Sequence Selectivity of DNA-Binding Molecules Using a Gold Nanoparticle-Based Colorimetric ApproachAnalytical Chemistry, 2007
- Analysis of Adenosine Triphosphate and Glutathione through Gold Nanoparticles Assisted Laser Desorption/Ionization Mass SpectrometryAnalytical Chemistry, 2007
- Nanoparticle Self-Assembly Gated by Logical Proteolytic TriggersJournal of the American Chemical Society, 2007
- The Aggregation of Au Nanoparticles by an Autonomous DNA Machine Detects VirusesSmall, 2007
- A Gold Nanoparticle Based Approach for Screening Triplex DNA BindersJournal of the American Chemical Society, 2006
- Sensitive Detection of Proteins Using Difunctional DNA–Gold NanoparticlesSmall, 2005
- Signaling Aptamers for Monitoring Enzymatic Activity and for Inhibitor ScreeningChemBioChem, 2004
- Tunable plasmonic glucose sensing based on the dissociation of Con A-aggregated dextran-coated gold colloidsAnalytica Chimica Acta, 2004