The detection of enteric viruses in selected urban and rural river water and sewage in Kenya, with special reference to rotaviruses
Open Access
- 16 August 2010
- journal article
- research article
- Published by Oxford University Press (OUP) in Journal of Applied Microbiology
- Vol. 109 (3), 818-828
- https://doi.org/10.1111/j.1365-2672.2010.04710.x
Abstract
Aim: To determine the occurrence of eight human enteric viruses in surface water and sewage samples from different geographical areas in Kenya. Methods and Results: Enteric viruses were recovered from the water and sewage sources by glass‐wool adsorption elution and/or polyethylene glycol/NaCl precipitation and detected by singleplex real‐time and conventional PCR and reverse transcriptase‐PCR assays. One or more enteric viruses were detected in nearly all sewage and river water samples except the urban Mbagathi River. The VP7 (G types) and the VP4 (P types) of the rotaviruses (RV) were characterized by multiplex nested PCR methods. The G and P types could be determined in 95·5% of the RV strains, respectively. Mixed G types were detected with G12 and G1 predominating, and unusual G types, G5 and G10, were present. P[4] predominated in the urban Karen sewage samples, while P[8] predominated in the urban and rural streams. Conclusions: The high prevalence of RVs in surface water highlights the importance of assessing the water sources used for domestic purposes for viral contamination. Significance and Impact of the Study: This study demonstrates the benefit of environmental surveillance as an additional tool to determine the epidemiology of RVs and other enteric viruses circulating in a given community.Keywords
This publication has 71 references indexed in Scilit:
- G2P[4] the most prevalent rotavirus genotype in 2007 winter season in an European non-vaccinated populationJournal of Clinical Virology, 2009
- New tools for the study and direct surveillance of viral pathogens in waterCurrent Opinion in Biotechnology, 2008
- Evaluation of Removal of Noroviruses during Wastewater Treatment, Using Real-Time Reverse Transcription-PCR: Different Behaviors of Genogroups I and IIApplied and Environmental Microbiology, 2007
- Sapovirus detection by quantitative real-time RT-PCR in clinical stool specimensJournal of Virological Methods, 2006
- Development, Evaluation, and Standardization of a Real-Time TaqMan Reverse Transcription-PCR Assay for Quantification of Hepatitis A Virus in Clinical and Shellfish SamplesApplied and Environmental Microbiology, 2006
- Enterically infecting viruses: pathogenicity, transmission and significance for food and waterborne infectionJournal of Applied Microbiology, 2005
- Detection of human enteric viruses in stream water with RT-PCR and cell cultureJournal of Water and Health, 2004
- Rotavirus infection in adultsThe Lancet Infectious Diseases, 2004
- Rapid and sensitive diagnosis of human adenovirus infections by a generic polymerase chain reactionJournal of Virological Methods, 2001
- Waterborne Viruses Associated With Hepatitis OutbreakJournal AWWA, 1991