Antifungal Resistance of Candida glabrata Vaginal Isolates and Development of a Quantitative Reverse Transcription-PCR-Based Azole Susceptibility Assay
- 1 September 2008
- journal article
- research article
- Published by American Society for Microbiology in Antimicrobial Agents and Chemotherapy
- Vol. 52 (9), 3424-3426
- https://doi.org/10.1128/aac.00462-08
Abstract
A multiplex quantitative reverse transcription-PCR assay was developed to detect azole resistance in Candida glabrata , an important opportunistic pathogen that develops resistance rapidly. Resistance was defined as a ≥3-fold increase in CDR1 expression by this assay, which proved to be 100% sensitive and 95% specific in comparison to the gold standard broth microdilution assay.Keywords
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