Longitudinal Study of the Molecular Epidemiology of Campylobacter jejuni in Cattle on Dairy Farms
- 15 June 2008
- journal article
- Published by American Society for Microbiology in Applied and Environmental Microbiology
- Vol. 74 (12), 3626-3633
- https://doi.org/10.1128/aem.01669-07
Abstract
Multilocus sequence typing (MLST), an accurate and phylogenetically robust characterization method for population studies of Campylobacter , was applied to Campylobacter jejuni isolates ( n = 297) from the fecal samples of cattle from five dairy farms in Cheshire, United Kingdom, collected throughout 2003. The population dynamics of the C. jejuni strains, as identified by the occurrence of sequence types and clonal complexes, demonstrated variations within and between cattle populations over time. Three clonal lineages have emerged to predominate among the cattle isolates, namely, the ST-61 complex (24.2%), ST-21 complex (23.6%), and ST-42 complex (20.5%). This provided further evidence that the ST-61 clonal complex may present a cattle-adapted C. jejuni genotype. In addition, the ST-42 clonal complex may also represent an important cattle-associated genotype. Strong geographical associations for these genotypes were also found among the farms. This is the first longitudinal study and the largest study to date for C. jejuni involving cattle populations using MLST for accurate strain characterization. This study shows the important associations between cattle and C. jejuni clonal complexes ST-61, ST-21, and ST-42, and it suggests that cattle and/or dairy products are likely to be a source of the human Campylobacter gastroenteritis caused by such genotypes. The reported findings have significant implications for the design of effective intervention strategies for disease control and prevention.Keywords
This publication has 36 references indexed in Scilit:
- Longitudinal Study of Finnish Campylobacter jejuni and C. coli Isolates from Humans, Using Multilocus Sequence Typing, Including Comparison with Epidemiological Data and Isolates from Poultry and CattleApplied and Environmental Microbiology, 2007
- Multilocus Sequence Typing Supports the Hypothesis that Cow- and Human-AssociatedSalmonellaIsolates Represent Distinct and Overlapping PopulationsApplied and Environmental Microbiology, 2006
- Campylobacter jejuniMultilocus Sequence Types in Humans, Northwest England, 2003––2004Emerging Infectious Diseases, 2006
- Long-lasting Campylobacter jejuni contamination of milk associated with gastrointestinal illness in a farming familyEpidemiology and Infection, 2005
- Spatial epidemiology and natural population structure of Campylobacter jejuni colonizing a farmland ecosystemEnvironmental Microbiology, 2005
- Applicability of a rapid duplex real-time PCR assay for speciation ofCampylobacter jejuniandCampylobacter colidirectly from culture platesFEMS Microbiology Letters, 2003
- Campylobacter jejuni—An Emerging Foodborne PathogenEmerging Infectious Diseases, 1999
- Bird attack on milk bottles: possible mode of transmission of Campylobacter jejuni to manThe Lancet, 1990
- Evidence of udder excretion ofCampylobacter jejunias the cause of milk-borne campylobacter outbreakEpidemiology and Infection, 1985
- Measurement of DiversityNature, 1949