Glial cells in the mouse enteric nervous system can undergo neurogenesis in response to injury
Open Access
- 1 September 2011
- journal article
- Published by American Society for Clinical Investigation in JCI Insight
- Vol. 121 (9), 3412-3424
- https://doi.org/10.1172/jci58200
Abstract
The enteric nervous system (ENS) in mammals forms from neural crest cells during embryogenesis and early postnatal life. Nevertheless, multipotent progenitors of the ENS can be identified in the adult intestine using clonal cultures and in vivo transplantation assays. The identity of these neurogenic precursors in the adult gut and their relationship to the embryonic progenitors of the ENS are currently unknown. Using genetic fate mapping, we here demonstrate that mouse neural crest cells marked by SRY box–containing gene 10 (Sox10) generate the neuronal and glial lineages of enteric ganglia. Most neurons originated from progenitors residing in the gut during mid-gestation. Afterward, enteric neurogenesis was reduced, and it ceased between 1 and 3 months of postnatal life. Sox10-expressing cells present in the myenteric plexus of adult mice expressed glial markers, and we found no evidence that these cells participated in neurogenesis under steady-state conditions. However, they retained neurogenic potential, as they were capable of generating neurons with characteristics of enteric neurons in culture. Furthermore, enteric glia gave rise to neurons in vivo in response to chemical injury to the enteric ganglia. Our results indicate that despite the absence of constitutive neurogenesis in the adult gut, enteric glia maintain limited neurogenic potential, which can be activated by tissue dissociation or injury.Keywords
This publication has 51 references indexed in Scilit:
- Enteric glia are multipotent in culture but primarily form glia in the adult rodent gutJCI Insight, 2011
- Prospective Identification and Isolation of Enteric Nervous System Progenitors Using Sox2The International Journal of Cell Cloning, 2010
- Directing Astroglia from the Cerebral Cortex into Subtype Specific Functional NeuronsPLoS Biology, 2010
- 5-HT4Receptor-Mediated Neuroprotection and Neurogenesis in the Enteric Nervous System of Adult MiceJournal of Neuroscience, 2009
- Far-red fluorescent tags for protein imaging in living tissuesBiochemical Journal, 2009
- Quantitative assessment of glial cells in the human and guinea pig enteric nervous system with an anti‐Sox8/9/10 antibodyJournal of Comparative Neurology, 2008
- Spatial organization and dynamic properties of neurotransmitter release sites in the enteric nervous systemNeuroscience, 2007
- Early Postnatal Astroglial Cells Produce Multilineage Precursors and Neural Stem CellsIn VivoJournal of Neuroscience, 2006
- Phenotypes of neural-crest-derived cells in vagal and sacral pathwaysCell and tissue research, 2005
- A single protocol to detect transcripts of various types and expression levels in neural tissue and cultured cells: in situ hybridization using digoxigenin-labelled cRNA probesHistochemistry and Cell Biology, 1993