Polyribosomes from Escherichia coli : Enzymatic Method for Isolation
- 6 August 1965
- journal article
- research article
- Published by American Association for the Advancement of Science (AAAS) in Science
- Vol. 149 (3684), 647-649
- https://doi.org/10.1126/science.149.3684.647
Abstract
Polyribosomes can be rapidly extracted from Escherichia coli by sequential passage of the cells through solutions of a chelating agent and lysozyme in a centrifugal field. Biosynthesis of protein in whole cells and in a cell-free system occurs almost exclusively in the polyribosomes.Keywords
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